Lipid-associated chlorophyll: evidence from 13C-NMR of the photosynthetic spinach thylakoid membrane

The 13C-NMR spectrum at 90.5 MHz has been obtained for the photosynthetic thylakoid membrane of spinach. Specific lipid and chlorophyll resonances can be assigned in the high resolution spectrum, although protein resonances are not observed. It can be estimated from resonance intensities that at lea...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 642; no. 2; p. 438
Main Authors Eigenberg, K E, Croasmun, W R, Chant, S I
Format Journal Article
LanguageEnglish
Published Netherlands 06.04.1981
Subjects
Chlorophyll - metabolism
Chloroplasts - metabolism
Intracellular Membranes - metabolism
Magnetic Resonance Spectroscopy
Membrane Lipids - metabolism
Photosynthesis
Vegetables
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Summary:The 13C-NMR spectrum at 90.5 MHz has been obtained for the photosynthetic thylakoid membrane of spinach. Specific lipid and chlorophyll resonances can be assigned in the high resolution spectrum, although protein resonances are not observed. It can be estimated from resonance intensities that at least 30% of the plant chlorophyll contributes to the high resolution 13C spectrum with the remainder broadened by incomplete motional averaging. The resonance linewidths of the observed chlorophyll phytol chains are approximately the same as those of the lipid hydrocarbon chains, indicating a similar motional state and suggesting that this particular pool of chlorophyll is lipid-bound or at most only loosely associated with proteins.
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ISSN:0006-3002
DOI:10.1016/0005-2736(81)90460-0