111Indium-F(ab)′ 2-NCA 102 monoclonal antibody: In vitro study of a specific agent for the detection of inflammatory foci

• Published in: Nuclear medicine and biology Vol. 20; no. 2; pp. 175 - 182
• Main Authors: Collet, B., Maros, S., Moisan, A., Le Cloirec, J., Moinereau, M., Aumaitre, E., Toujas, L., Bourguet, P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.1993
• Subjects: Animals; Antibodies, Monoclonal; Blood - diagnostic imaging; Bone Marrow - ultrastructure; Granulocytes - metabolism; Humans; Indium Radioisotopes; Inflammation - diagnostic imaging; Liver - ultrastructure; Mice; Mice, Inbred BALB C; Radionuclide Imaging
• ISSN: 0969-8051; 1872-9614
• DOI: 10.1016/0969-8051(93)90112-8

A new antigranulocyte antibody was evaluated in vitro for the detection of inflammatory foci in man. The specificity for polymorphonuclear cells (PMN) of NCA 102, an anti-NCA 95 monoclonal IgG1, was determined with immunohistochemical and cytofluorometrical tests. Its affinity, assessed by Scatchard analysis, was 1.1 × 10 9L/mol and the number of epitopes per granulocyte reached about 10 5. The biological properties of PMNs incubated with NCA 102 were not inhibited even when coupled with DTPA. A F(ab)′ 2 fragment was radiolabelled with 111Indium and incubated in the presence of whole blood. More than 65% radioactivity was selectively taken up by the PMN population. These findings indicated that NCA 102 antibody is suitable for sepsis detection.