CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes

• Published in: Nephrology, dialysis, transplantation Vol. 14; no. 6; pp. 1430 - 1434
• Main Authors: RENTENAAR, R. J, WEVER, P. C, VAN DIEPEN, F. N. J, SCHELLEKENS, P. T. A, WERTHEIM, P. M. E, TEN BERGE, I. J. M
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.1999
• Subjects: Biological and medical sciences; CD4 Antigens - analysis; CD8 Antigens - analysis; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Granzymes; Humans; Immunobiology; Immunologic Memory; Immunophenotyping; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Medical sciences; Middle Aged; Serine Endopeptidases - metabolism; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the urinary system; T-Lymphocytes - immunology; Flow cytometry; Homograft; Cellular immunity; Transplantation; Betaherpesvirinae; Kidney; Granzyme B; Surgery; T-Lymphocyte; Adult; Female; Quantitative analysis; Human; Cytomegalovirus; Serine endopeptidases; Enzyme; Herpesviridae; Gene expression; Cell subpopulation; Virus; Case study; Infection; Peptidases; Phenotype; Viral disease; Hydrolases
• ISSN: 0931-0509; 1460-2385; 1460-2385
• DOI: 10.1093/ndt/14.6.1430

T-lymphocytes that co-express CD4 and CD8 antigens may be found in small percentages in the peripheral blood of healthy individuals, and have a CD4brightCD8dull phenotype. CD4dullCD8bright T-lymphocytes have been found only in temporal association with some viral infections. Four-colour flow cytometric analysis of peripheral blood mononuclear cells from a renal transplant recipient with cytomegalovirus infection was performed. A small but clearly distinguishable subpopulation of CD4dullCD8bright double-positive T-lymphocytes was detected, that exhibited phenotypic characteristics of cytotoxic T-lymphocytes and were granzyme B positive. Furthermore, no naive cells appeared to be present within this subpopulation. CD4dullCD8bright double-positive T-lymphocytes are enriched for memory and effector cytotoxic T cells.