2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) Alters the Regulation and Posttranslational Modification of p27kip1 in Lipopolysaccharide-Activated B Cells

• Published in: Toxicological sciences Vol. 75; no. 2; pp. 333 - 342
• Main Authors: Crawford, Robert B., Sulentic, Courtney E. W., Yoo, Byung S., Kaminski, Norbert E.
• Format: Journal Article
• Language: English
• Published: Cary, NC Oxford University Press 01.10.2003
• Subjects: Animals; B cell; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Biological and medical sciences; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Line, Tumor; Chemical and industrial products toxicology. Toxic occupational diseases; cyclin-dependent kinase inhibitor; Cyclin-Dependent Kinase Inhibitor p27; Dose-Response Relationship, Drug; Environmental Pollutants - toxicity; Genes, Tumor Suppressor; Lipopolysaccharides - pharmacology; LPS; Lymphocyte Activation - drug effects; Medical sciences; Mice; p27kip1; Polychlorinated Dibenzodioxins - toxicity; Protein Processing, Post-Translational - drug effects; TCDD; Toxicology; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; Various organic compounds; Toxicity; TCDD; cyclin-dependent kinase inhibitor; LPS; Posttranslational modification; B cell; p27kip1; Chlorine Organic compounds; Inhibition; Mechanism of action; Immune system; Immunopathology; Immune response; Enzyme; Transferases; Rodentia; Enzyme inhibitor; B-Lymphocyte; Cell differentiation; In vitro; Pollutant; Vertebrata; Mammalia; Mouse; Protein kinase; Animal
• ISSN: 1096-6080; 1096-0929; 1096-0929
• DOI: 10.1093/toxsci/kfg199

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) alters B-cell differentiation, as evidenced by a marked decrease in immunoglobulin M (IgM) secretion and in the number of antibody-forming cells (AFC) induced by antigenic stimulation. The objective of the present studies was to evaluate the effect of TCDD on the level of p27kip1, a cyclin-dependent kinase inhibitor that is a critical regulator of cellular differentiation. In the well-characterized B-cell line, CH12.LX, a modest decrease in p27kip1was observed during the initial 24-h post-LPS (lipopolysaccharide) activation, which then gradually increased above background at 48 and 72 h. Conversely, in the presence of TCDD, p27kip1was not induced and remained unchanged from LPS unstimulated cells throughout the entire 72-h period post-LPS activation. In addition, Western blotting revealed that TCDD treatment altered the profile of p27kip1 migration as compared to the LPS-activated control. Time-of-addition studies demonstrated that the greatest sensitivity of p27kip1 to TCDD treatment occurred within the initial 24-h post-LPS activation. Interestingly, LPS-induced Ig κ light chain and IgM secretion also exhibited the greatest period of sensitivity (i.e., inhibition) to TCDD during the first 24-h post-LPS activation. In addition, TCDD markedly suppressed the LPS-induced differentiation of CH12.LX cells into IgM secreting AFC, with a modest but cumulative effect on cell proliferation over a 72-h period. Collectively, these findings show that TCDD altered the cellular concentration and posttranslational modification of p27kip1 in this activated B-cell line model, which occurred concomitantly with altered B-cell differentiation and suggests that cyclin-dependent kinase inhibitors may be an important intracellular target in TCDD-mediated inhibition of B-cell differentiation.