Proton gradient across membranes of lecithin vesicles as measured by 31P-NMR: Asymmetrical behaviour of internal and external layers

The ionization of phosphate ions trapped inside sonicated egg yolk lecithin vesicles was determined as a function of internal pH values using a 31PNMR. The chemical shift of the internal phosphate signal reflects the intravesicular pH perfectly. At 30°C, protons once inside vesicles (pH int < pH...

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Published inBiochemical and biophysical research communications Vol. 95; no. 3; pp. 1218 - 1223
Main Authors Prigent, Yves, Trandinh, Son, Gary-Bobo, Claude M.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 14.08.1980
Subjects
Biological Transport
Hydrogen-Ion Concentration
Liposomes
Magnetic Resonance Spectroscopy
Models, Biological
Phosphates
Phosphatidylcholines
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Summary:The ionization of phosphate ions trapped inside sonicated egg yolk lecithin vesicles was determined as a function of internal pH values using a 31PNMR. The chemical shift of the internal phosphate signal reflects the intravesicular pH perfectly. At 30°C, protons once inside vesicles (pH int < pH ext) cannot get across the inner layer even under a pH gradient up to five units. On the other hand, when pH (int) >pH(ext), the results suggest that protons may cross the outer but not the inner layer and interact with the internal phosphate ions.
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(80)91603-4