Bioproduction of D-psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

• Published in: Frontiers of chemical engineering in China Vol. 3; no. 4; pp. 393 - 398
• Main Authors: Zhang, Longtao, Jiang, Bo, Mu, Wanmeng, Zhang, Tao
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Higher Education Press 01.12.2009
• Subjects: Chemistry; Chemistry and Materials Science; Industrial Chemistry/Chemical Engineering; Nanotechnology; Research Article; psicose; bioproduction; permeabilized cells
• ISSN: 1673-7369; 1673-7474
• DOI: 10.1007/s11705-009-0252-z

D -psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of D -psicose from D -fructose. In this paper, another bacterium which could convert D -fructose to D -psicose was isolated and identified as Rhodobacter sphaeroides . The process parameters of D -psicose production using permeabilized cells of Rhodobacter sphaeroides SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10 min, and reaction conditions: cell concentration, 30 g dry cell wt/L; concentration of substrate, 50 g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5 g/L D -psicose with a D -psicose productivity of 0.82 g·L −1 ·h −1 . This is the first report of bioproduction of D -psicose using permeabilized cells of Rhodobacter sphaeroides .