Bioproduction of D-psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011
D -psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of D -psicose from D -fructose. In this paper, another bacterium which could convert D -fructose to D -psicose was isolated and identifie...
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Published in | Frontiers of chemical engineering in China Vol. 3; no. 4; pp. 393 - 398 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
SP Higher Education Press
01.12.2009
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Subjects | |
Online Access | Get full text |
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Summary: | D
-psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of
D
-psicose from
D
-fructose. In this paper, another bacterium which could convert
D
-fructose to
D
-psicose was isolated and identified as
Rhodobacter sphaeroides
. The process parameters of
D
-psicose production using permeabilized cells of
Rhodobacter sphaeroides
SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10 min, and reaction conditions: cell concentration, 30 g dry cell wt/L; concentration of substrate, 50 g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5 g/L
D
-psicose with a
D
-psicose productivity of 0.82 g·L
−1
·h
−1
. This is the first report of bioproduction of
D
-psicose using permeabilized cells of
Rhodobacter sphaeroides
. |
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ISSN: | 1673-7369 1673-7474 |
DOI: | 10.1007/s11705-009-0252-z |