Effect of Host Plants on Insecticide Susceptibility and Detoxification Enzymes Activity in Spodoptera litura Fabricius (Noctuidae: Lepidoptera)
Susceptibility to two insecticides (profenophos and cypermethrin) as influenced by host plants viz., cauliflower, soybean and castor in comparison to artificial diet and changes in detoxification enzymes in tobacco caterpillar, Spodoptera litura were studied under laboratory. In comparison to larvae...
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Published in | Proceedings of the National Academy of Sciences, India. Section B: Biological sciences Vol. 86; no. 3; pp. 715 - 721 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New Delhi
Springer India
01.09.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Susceptibility to two insecticides (profenophos and cypermethrin) as influenced by host plants viz., cauliflower, soybean and castor in comparison to artificial diet and changes in detoxification enzymes in tobacco caterpillar,
Spodoptera litura
were studied under laboratory. In comparison to larvae reared on artificial diet, those reared on cauliflower were tolerant to profenophos and cypermethrin by, 1.41 and 6.6 respectively, while the larvae reared on soybean showed 1.32 and 2.00-fold more tolerance to these insecticides. However, the larvae reared on castor were found to be more susceptible to profenophos than those reared on artificial diet. The host plants had a significant influence on levels of detoxification enzymes in
S. litura
. Elevated levels of carboxylesterase (20.72-fold) and cytochrome P450 mono-oxygenase (3.10-fold) were observed in larvae reared on cauliflower as compared to diet fed larvae. Activity of glutathione S-transferase (1.56-fold) was higher in larvae reared on soybean. Enhanced activity of detoxification enzymes in larvae of
S. litura
reared on different host plants could be correlated with insecticide susceptibility. Positive and significant (p < 0.05) correlation was observed between the detoxification enzyme (CarE and GST) activity in different host fed larvae and LD
50
of profenophos (r = 0.77; r = 0.93). Activity of cytochrome P450 mono-oxygenase also had positive correlation with LD
50
of cypermethin (r = 0.98). The present studies have shown that differential susceptibility of
S. litura
population could be correlated with the diet/host plants and levels of detoxifications enzymes like carboxylesterases, glutathione S-transferase and cytochrome P450 mono-oxygenase. |
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ISSN: | 0369-8211 2250-1746 |
DOI: | 10.1007/s40011-015-0515-z |