Use of RAPD and ITE molecular markers in studying the genetic structure of the Crimean population of T. boeoticum Boiss

• Published in: Cytology and genetics Vol. 41; no. 3; pp. 181 - 189
• Main Authors: Mallabaeva, D. Sh, Ignatov, A. N., Sheiko, I. A., Isikov, V. P., Gelyuta, V. P., Boiko, N. G., Seryapin, A. A., Dorokhov, D. B.
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01.06.2007
• Subjects: Climatic conditions; Genetic structure; Natural selection; Random amplified polymorphic DNA; Triticum boeoticum
• ISSN: 0095-4527; 1934-9440
• DOI: 10.3103/S0095452707030085

The influence of ecological factors on variation of random PCR markers (RAPD and inter-MITE polymorphism (IMP) primers) was evaluated in two wild Triticum boeoticum populations with contrasting climatic conditions in Crimea. The proportion of variation that undergoes natural selection was compared for these two types of molecular markers. The Sapun Mountain and Baidar Valley populations differed significantly in 24.7% of the analyzed loci, with a low Nei distance between these populations (0.0324). The number of significantly different RAPD and IMP markers in the two populations was nearly equal. Testing for evolutionary neutrality showed that 13 loci (17.8%) were influenced by natural selection. The Sapun Mountain population was more dependent on natural selection (ten loci, 13.7%) than the Baidar Valley population (four loci, 5.5%). Eleven out of the 13 loci undergoing selection were amplified with IMP primers.