Successful semen collection and cryopreservation of Banteng semen (Bos javanicus) using an artificial vagina

Abstract Gamete collection is feasible in Banteng ( Bos javanicus ) and is essential for conserving this species. The common practice of semen collection in wild animals is using the electroejaculation technique which is considered invasive with lower-quality results. This study aims to develop sper...

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Bibliographic Details
Published inIOP conference series. Earth and environmental science Vol. 1109; no. 1; pp. 12095 - 12101
Main Authors Setiadi, D R, Mulia, B H, Widianti, A, Manansang, J, Hastuti, Y T, Yoelinda, V T, Agil, M, Arifiantini, R I, Nugraha, T P
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.11.2022
Subjects
Acclimatization
Bos javanicus
Collection
Cryopreservation
Freezing
Goats
Liquid nitrogen
Motility
Semen
Sperm
Thawing
Vagina
Wild animals
Wildlife tourism
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Summary:Abstract Gamete collection is feasible in Banteng ( Bos javanicus ) and is essential for conserving this species. The common practice of semen collection in wild animals is using the electroejaculation technique which is considered invasive with lower-quality results. This study aims to develop sperm collection further using an artificial vagina and to investigate the sperm motility of Banteng after being frozen-thawed. A Banteng bull kept in Taman Safari Indonesia was used for this study. Before semen collection, the bull is acclimatized to collection conditions. Semen collection is done once a month for six months. The semen was evaluated according to a method for bovine semen. Cryopreservation of semen was performed using an Andromed ® extender, in 0.25 mL Ministraw ® , and equilibrated at 4°C for 3 hours, followed by freezing in liquid nitrogen vapor. Quality testing was performed using the CASA IVOS II™. The result showed variations between batches, with the number of mountings 9.83±2.86(7-15) times, semen volume 8.42±1.42 (6-9) mL, straw produced 152±15.23 (130-172) straws, and total motility after thawing 44.28 ±12.14, (26.83-55.96). The quality of frozen semen of the later batch showed better results p<0.05. In conclusion, the collection of Banteng semen using an artificial vagina showed promising results with moderate quality.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/1109/1/012095