Production of laccase and manganese peroxidase by white-rot fungi from sugarcane bagasse in solid bed: Use for dyes decolourisation

• Published in: Sugar tech : an international journal of sugar crops & related industries Vol. 10; no. 3; pp. 260 - 264
• Main Authors: Guerra, Gilda, Domínguez, Osmel, Ramos-Leal, Miguel, Manzano, Ana M., Sánchez, María I., Hernández, Ianeya, Palacios, Jorge, Arguelles, Juan
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.10.2008
• Subjects: Agriculture; Biomedical and Life Sciences; Life Sciences; Research Article; white-rot fungi; ligninolytic enzymes; dye biotransformation; Sugarcane bagasse
• ISSN: 0972-1525; 0974-0740
• DOI: 10.1007/s12355-008-0046-5

Three strains of white-rot fungi were tested in solid state fermentation and submerged culture to obtain enzymes for dyes biotransformation. Both sugarcane bagasse and dyes induced laccase and manganese peroxidase biosynthesis but laccase seems to be the main enzyme related to the decolourisation profiles. A variable behavior of strains was observed depending on inducers, fermentation system and characteristics of the strains. Crude enzyme of Earliella scabrosa obtained in solid state fermentation showed higher decolourisation percentage of Navy FNB and Red FN-3G dyes than Trametes maxima (13) and Ganoderma zonatum (B-18). T. maxima exhibited the best decolourisation percentage in submerged cultures supplemented with Navy FNB, Red FN- 3G and yellow P-6GS dyes. Growing biomass of T. maxima could supply other enzymes and mediators for dyes transformation. Peculiar behaviour was observed with Ganoderma zonatum (B-18), it had a similar dyes biodegradation in both liquid and solid bed fermentation and there was not positive correlation between ligninolytic enzymes production and decolourisation pattern. The employment of crude enzymes produced in solid bed of bagasse could be an attractive option for biological removal of textile dyes.