Molecular biological properties of new isolates of plum pox virus strain Winona
Plum pox virus (PPV, genus Potyvirus , family Potyviridae ) is the most important virus pathogen of stone fruit cultures of the genus Prunus from an economical standpoint. Winona strain (PPV-W) is the most variable of nine known virus strains and one of the most widespread in the European part of Ru...
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Published in | Moscow University biological sciences bulletin Vol. 71; no. 2; pp. 71 - 75 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New York
Allerton Press
01.04.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Plum pox virus (PPV, genus
Potyvirus
, family
Potyviridae
) is the most important virus pathogen of stone fruit cultures of the genus
Prunus
from an economical standpoint. Winona strain (PPV-W) is the most variable of nine known virus strains and one of the most widespread in the European part of Russia. Six new PPV-W isolates were first discovered in green plantations of Moscow (Kp2U, Avang, Pulk, Pulk-1), in Taldomsky district of Moscow region (Karm), and in Kovrovsky district of Vladimir region (Vlad-4) on wild trees of plum
Prunus domestica
. 3’-Terminal segment of genome of new isolates was notable for high variability level. The study on the relationship with other isolates of this strain by means of phylogenetic analysis of gene sequence of the coat protein showed the lack of clusterization of Russian PPV-W isolates according to geographical principle. Inoculation of
Nicotiana benthamiana
plants by hop plant louse
Phorodon humili
from plum trees infected with Avang and Pulk isolates and by thistle aphid
Brachycaudus cardui
from the tree infected with Kp2U isolate led to the systemic viral infection in indicator plants, suggesting the possibility of PPV-W spread by both aphid species in nature. Transmission of PPV-W through seeds was not observed. |
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ISSN: | 0096-3925 1934-791X |
DOI: | 10.3103/S0096392516020103 |