Thromboplastin and plasminogen activator activities of human leukemic cell lines

Nine leukemic cell lines, KML-1 (APL), PL-21 (APL), HL-60 (APL), ML-1 (AML), KCL-22 (CML), KEN-L-1 (common ALL), NALM-18 (pre-B cell ALL), TALL-1 (T-cell ALL) and RC-K8 (histiocytic lymphoma), were investigated about their thromboplastin (TP) and plasminogen activator (PA) activities by the method b...

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Bibliographic Details
Published inBlood & Vessel Vol. 16; no. 6; pp. 637 - 639
Main Authors NIIYA, Kenji, KUBONISHI, Ichiro, MIYOSHI, Isao
Format Journal Article
LanguageEnglish
Published The Japanese Society on Thrombosis and Hemostasis 1985
Subjects
leukemic cell line
plasminogen activator
SDS-PAGE
thromboplastin
zymography
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Summary:Nine leukemic cell lines, KML-1 (APL), PL-21 (APL), HL-60 (APL), ML-1 (AML), KCL-22 (CML), KEN-L-1 (common ALL), NALM-18 (pre-B cell ALL), TALL-1 (T-cell ALL) and RC-K8 (histiocytic lymphoma), were investigated about their thromboplastin (TP) and plasminogen activator (PA) activities by the method based on factor Xa generation in the presence of factors VII and X using chromogenic substrate S2222 and fibrin-agar method, respectively. The granulocytic cell lines such as ML-1, HL-60, and PL-21 were found to have significant TP activity in their cell lysates. The cell lines including RC-K8, HL-60, NALM-18 and KML-1 had significant PA activity in their cell lysates. A large amount of PA activity was found in the culture medium from RC-K8. The PA from RC-K8 was immunologically identical to urokinase and showed two different fibrinolytic bands, molecular weights of 58, 000 and 53, 000 on SDS-PAGE followed by zymography. Therefore, RC-K8 seems to be useful as one of the PA-producing cell lines.
ISSN:0386-9717
1884-2372
DOI:10.2491/jjsth1970.16.637