A Single Extracellular Vesicle (EV) Flow Cytometry Approach to Reveal EV Heterogeneity

Extracellular vesicles (EVs) actively participate in intercellular communication and pathological processes. Studying the molecular signatures of EVs is key to reveal their biological functions and clinical values, which, however, is greatly hindered by their sub‐100 nm dimensions, the low quantitie...

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Published inAngewandte Chemie Vol. 130; no. 48; pp. 15901 - 15906
Main Authors Shen, Wen, Guo, Kaizhu, Adkins, Gary Brent, Jiang, Qiaoshi, Liu, Yang, Sedano, Sabrina, Duan, Yaokai, Yan, Wei, Wang, Shizhen Emily, Bergersen, Kristina, Worth, Danielle, Wilson, Emma H., Zhong, Wenwan
Format Journal Article
LanguageEnglish
Published Weinheim Wiley Subscription Services, Inc 26.11.2018
Subjects
Bioanalytische Chemie
Biomedical materials
Biomolecules
Breast cancer
Cancer
Cell signaling
Chemistry
Deoxyribonucleic acid
DNA
Extrazelluläre Vesikel
Flow cytometry
Flusszytometrie
Heterogeneity
Heterogenität
Molekulare Signaturen
Phenotyping
Signatures
Statistical analysis
Tumor cell lines
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Summary:Extracellular vesicles (EVs) actively participate in intercellular communication and pathological processes. Studying the molecular signatures of EVs is key to reveal their biological functions and clinical values, which, however, is greatly hindered by their sub‐100 nm dimensions, the low quantities of biomolecules each EV carries, and the large population heterogeneity. Now, single‐EV flow cytometry analysis is introduced to realize single EV counting and phenotyping in a conventional flow cytometer for the first time, enabled by target‐initiated engineering (TIE) of DNA nanostructures on each EV. By illuminating multiple markers on single EVs, statistically significant differences are revealed among the molecular signatures of EVs originating from several breast cancer cell lines, and the cancer cell‐derived EVs among the heterogeneous EV populations are successfully recognized. Thus, our approach holds great potential for various biological and biomedical applications. Flusszytometrie einzelner extrazellulärer Vesikel (EV) durch targetinitiierte Modifizierung (TIE) von DNA‐Nanostrukturen ermöglicht die Identifizierung einzigartiger molekularer Signaturen individueller Vesikel und die Differenzierung von EV‐Unterpopulationen.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201806901