Disruption of Imprinted Expression ofU2afbp-rs/U2af1-rs1 Gene in Mouse Parthenogenetic Fetuses

The present study shows that theU2afbp-rs gene, a paternally expressed imprinted gene, is activated and expressed in a biallelic manner from maternal alleles in parthenogenetic mouse fetuses on day 9.5 of gestation. The mean expression was detected to be 88% (31–134%) of that in control biparental f...

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Published inThe Journal of biological chemistry Vol. 276; no. 28; pp. 26694 - 26698
Main Authors Sotomaru, Yusuke, Kawase, Yosuke, Ueda, Takayuki, Obata, Yayoi, Suzuki, Hiroshi, Domeki, Ikuo, Hatada, Izuho, Kono, Tomohiro
Format Journal Article
LanguageEnglish
Published Elsevier Inc 13.07.2001
American Society for Biochemistry and Molecular Biology
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Summary:The present study shows that theU2afbp-rs gene, a paternally expressed imprinted gene, is activated and expressed in a biallelic manner from maternal alleles in parthenogenetic mouse fetuses on day 9.5 of gestation. The mean expression was detected to be 88% (31–134%) of that in control biparental fetuses, using real-time quantitative reverse transcription and polymerase chain reaction. This disrupted expression of the gene was associated with changes in the chromatin structure but not with the methylation pattern in the regulation region. The present results show that parental specific expression of imprinted genes is not always maintained in uniparental embryos. This suggests that both parental genomes are necessary to establish parental specific expression of theU2afbp-rs gene.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M101367200