Induction and Characterization of Chromosome-doubled Lavandin (Lavandula × intermedia Emeric ex Loisel.) by Colchicine Treatment of Leaf-derived Callus
In order to develop an efficient procedure for chromosome doubling of lavandin, leaf-derived calli were treated with colchicine. Relatively high adventitious shoot formation rates, from 71.8 to 79.3%, were obtained in the medium with 0.05, 0.1 and 0.5% of colchicine, although almost all shoots showe...
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Published in | Engeigaku kenkyuu Vol. 10; no. 3; pp. 303 - 308 |
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Main Authors | , , , |
Format | Journal Article |
Language | Japanese English |
Published |
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
2011
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Subjects | |
Online Access | Get full text |
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Summary: | In order to develop an efficient procedure for chromosome doubling of lavandin, leaf-derived calli were treated with colchicine. Relatively high adventitious shoot formation rates, from 71.8 to 79.3%, were obtained in the medium with 0.05, 0.1 and 0.5% of colchicine, although almost all shoots showed hyperhydricity. However, root formation rates were seriously decreased. Conclusively, 2, 16 and 7 chromosome-doubled plants were obtained from calli treated with 0.05, 0.1 and 0.5% of colchicine, where the regeneration rates were 0.6, 4.3 and 2.0%, respectively. The leaf lengths of chromosome-doubled plants varied widely, and the stomatal size was significantly larger than that of the original. However, both the floral stalks and spikes of chromosome-doubled plants showed dwarfism. Additionally, the essential oil contents in the floral spikes of chromosome-doubled plants significantly decreased. When the crossability is recognized between chromosome-doubled plants and true lavender, these amphidiploids might be interesting materials for improving horticultural traits of lavender. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1347-2658 1880-3571 |
DOI: | 10.2503/hrj.10.303 |