Induction and Characterization of Chromosome-doubled Lavandin (Lavandula × intermedia Emeric ex Loisel.) by Colchicine Treatment of Leaf-derived Callus

• Published in: Engeigaku kenkyuu Vol. 10; no. 3; pp. 303 - 308
• Main Authors: Tsuro, Masato, Ito, Yoshie, Morisue, Tomomi, Nakao, Yoshinori
• Format: Journal Article
• Language: Japanese; English
• Published: THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 2011
• Subjects: Callus; chromosome doubling; Chromosomes; Colchicine; dwarfing; Dwarfism; essential oil; Essential oils; Lavandula; Leaves; Roots; Shoots; Stomata
• ISSN: 1347-2658; 1880-3571
• DOI: 10.2503/hrj.10.303

In order to develop an efficient procedure for chromosome doubling of lavandin, leaf-derived calli were treated with colchicine. Relatively high adventitious shoot formation rates, from 71.8 to 79.3%, were obtained in the medium with 0.05, 0.1 and 0.5% of colchicine, although almost all shoots showed hyperhydricity. However, root formation rates were seriously decreased. Conclusively, 2, 16 and 7 chromosome-doubled plants were obtained from calli treated with 0.05, 0.1 and 0.5% of colchicine, where the regeneration rates were 0.6, 4.3 and 2.0%, respectively. The leaf lengths of chromosome-doubled plants varied widely, and the stomatal size was significantly larger than that of the original. However, both the floral stalks and spikes of chromosome-doubled plants showed dwarfism. Additionally, the essential oil contents in the floral spikes of chromosome-doubled plants significantly decreased. When the crossability is recognized between chromosome-doubled plants and true lavender, these amphidiploids might be interesting materials for improving horticultural traits of lavender.