HLA-B27 subtyping by PCR-RFLP in Spanish patients with ankylosing spondyiitis

• Published in: Tissue antigens Vol. 52; no. 5; pp. 492 - 496
• Main Authors: Fraile, A., Martin, J., Löpez-Nevot, M.A., Mataran, L., Nieto, A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.1998
• Subjects: ankylosing spondyiitis; HLA-B27 subtypes; PCR-RFLP
• ISSN: 0001-2815; 1399-0039
• DOI: 10.1111/j.1399-0039.1998.tb03078.x

: We describe the use of restriction analysis on PCR‐amplified DNA for detecting all B*27 subtypes except B*2710 and B*2711 (i.e. from B*2701 to B*2709). After detecting B*27 by Sty I, double digestions consisting of Sty I plus another informative enzyme led to subtype assignment. We used mismatched primers to create restriction sites when necessary. The method avoids group‐specific amplifications and other laborious optimization procedures. It was successfully tested on a panel of well characterized cell lines covering different B*27 subtypes. Then, we studied a group of 57 ankylosing spondyiitis patients and 746 controls from the south of Spain. B*27 showed a very strong association with the disease (OR=211.27, P=\0˜7). B*2702 and B*2705 distribution in controls (20% and 77.1%, respectively) differed from previously reported data in the Spanish population. We unexpectedly found the B*2707 allele in our population (one control).