Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using 1H NMR spectroscopy and multivariate statistical analysis

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 93; pp. 51 - 58
• Main Authors: Chen, Yan-Gan, Song, Yue-Lin, Wang, Ying, Yuan, Yun-Fei, Huang, Xiao-Jun, Ye, Wen-Cai, Wang, Yi-Tao, Zhang, Qing-Wen
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.05.2014
• Subjects: China; Discriminant Analysis; Isoflavones - chemistry; Isoflavones - isolation & purification; Least-Squares Analysis; Multivariate Analysis; Nuclear magnetic resonance; Plant Roots; Principal Component Analysis; Proton Magnetic Resonance Spectroscopy - methods; Pueraria - chemistry; Pueraria lobata; Pueraria thomsonii; Puerariae Radix; Puerarin; Quality Control; Species Specificity; China; Puerariae Radix; Pueraria thomsonii; Nuclear magnetic resonance; Pueraria lobata; Puerarin
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2013.05.017

•NMR spectroscopy based metabolomic study of Puerariea Radix was carried out.•P. lobata and P. thomsonii were discriminated markedly by either PCA or PLS-DA.•Isoflavones were screened out as the chemical markers to discriminate PL and PT.•The contents of puerarin and total isoflavones were determined by q1H NMR. Puerariae Radix was a widely used herbal medicine. Pueraria lobata (PL) and Pueraria thomsonii (PT) were the two authorized sources of Puerariae Radix (gegen) in China. In this study, metabolic differentiations between these two species were investigated using NMR spectroscopy followed by principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). The content of puerarin in PL and PT was also determined using quantitative 1H NMR spectroscopy. Thirteen isoflavones were tentatively identified based on 1D and 2D NMR spectroscopic data in two species. The 1H NMR spectra of PL and PT were obviously different. PL and PT could also be markedly discriminated from 1H NMR spectroscopic data by PCA and PLS-DA. For the crude drug resources, isoflavones, in which puerarin is the most important one, were regarded as the reasonable markers for the discrimination of the two species. The contents of puerarin and total isoflavones in PL were quantitated much higher than those in PT. Above all, 1H NMR spectroscopy, which can provide comprehensive profiles of the metabolites and achieve convenient determinations of puerarin and total isoflavones in a single run, is an efficient means for evaluating the medicinal samples and achieving a better quality control of Puerariae Radix.