Molecular identification and phylogenetic analysis of Pseudomonas sp. and Aeromonas sp. isolated from mine drainage water in Slovinky and Markušovce (Slovakia)

• Published in: Biológia Vol. 77; no. 11; pp. 3327 - 3332
• Main Authors: Perháčová, Zuzana, Prepilková, Veronika, Schwarz, Marián, Pristaš, Peter, Kisková, Jana
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.11.2022; Springer Nature B.V
• Subjects: Aeromonas; Bacteria; Biomedical and Life Sciences; Bioremediation; Biotechnology; Cell Biology; Drainage water; Genetic analysis; Genetic variability; Life Sciences; Microbial activity; Microbiology; Microorganisms; Mine drainage; Mine tailings; Phylogeny; Plant Sciences; Pseudomonas; Pseudomonas plecoglossicida; rRNA; Sequence analysis; Short Communication; Similarity; Zoology; Tailings pond; sp; Mine drainage water; 16S rRNA gene
• ISSN: 1336-9563; 0006-3088; 1336-9563
• DOI: 10.1007/s11756-022-01170-z

Mining areas are characterized by extreme conditions significantly affecting the diversity of microbial communities. On the other side, mining environments can be sources of bacteria with various biotechnological applications. In this study, two bacterial isolates from mine drainage water of two tailings ponds Slovinky and Markušovce (Slovakia) were identified using the 16 S rRNA gene sequence analysis. The isolate ODK1 found in mine drainage water from Slovinky showed a 99.73% similarity to the 16 S rDNA sequence of Pseudomonas plecoglossicida strain FPC951 (NR_024662.1) deposited in the GenBank database. The phylogenetic analysis showed a high genetic variability among Pseudomonas plecoglossicida group. The isolate ODK2 found in mine drainage water from Markušovce showed a 99.93% similarity to the 16 S rDNA sequence of Aeromonas salmonicida (NR_118945.1). The phylogenetic analysis confirmed their close relationship. Today, Pseudomonas spp., as well as Aeromonas spp. represent bacteria with a high bioremediation potential.