In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
Puya chilensis is an endemic species of Chile belonging to the Bromeliaceae family, known for its morphological plasticity and ecological importance. In recent years, greater attention has been given to its conservation due to the indiscriminate use of its populations for food and medicinal purposes...
Saved in:
Published in | Plant cell, tissue and organ culture Vol. 158; no. 2; p. 25 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.08.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Puya chilensis
is an endemic species of Chile belonging to the Bromeliaceae family, known for its morphological plasticity and ecological importance. In recent years, greater attention has been given to its conservation due to the indiscriminate use of its populations for food and medicinal purposes.
In vitro
culture is a tool for the propagation of plants species, as well as for obtaining bioactive compounds. In Chile,
P. chilensis
is the most representative within the
Puya
genus. This study focused on establishing an
in vitro
propagation protocol for
P. chilensis
that allows massive propagation and obtaining proteolytic enzymes. The combined effect of plants cuts and the application of 0.5 µmol L
−1
of BAP and GA
3
favored the
in vitro
multiplication of
P. chilensis
. Culture in liquid medium induced greater morphological development and early differentiation of anatomical structures in the leaves of
P. chilensis in vitro
. The use of TIS creates ideal conditions during the last stage of
in vitro
culture and ensures 100% survival during acclimatization phase. The management of cultivation conditions and the efficient use of TIS allowed the generation of
P. chilensis
plants with an optimal degree of development for obtaining proteolytic extracts. The main enzymes present in the extracts of
P. chilensis
plants grown
in vitro
belong to the cysteine type. This study proposes for the first time an optimized protocol for the propagation and conservation of
P. chilensis,
enhancing its uses as a source of biologically active molecules for the biotechnology and pharmaceutical industries.
Graphical Abstract |
---|---|
ISSN: | 0167-6857 1573-5044 |
DOI: | 10.1007/s11240-024-02811-8 |