Phytochemical effects of genistein and daidzein on sex hormones and corticosterone in female adult rats exposed to Chlorpyrifos

• Published in: Toxicology and environmental health sciences Vol. 14; no. 3; pp. 261 - 267
• Main Authors: Abdulretha, Shigaf D., Abdullah, Asia S., AL‑Mozie’l, Muhsin S. G.
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.09.2022; Springer Nature B.V
• Subjects: Biochemical analysis; Biochemical tests; Biomedical and Life Sciences; Biomedicine; Body weight; Chlorpyrifos; Corticosterone; Crop residues; Daidzein; Environmental Health; Females; Follicle-stimulating hormone; Genistein; Histopathology; Hormones; Insecticides; Isoflavones; Lipids; Luteinizing hormone; Oils & fats; Original Article; Ovaries; Oxidative stress; Peroxides; Pesticide toxicity; Pesticides; Pharmacology/Toxicology; Reproduction; Rodents; Sex hormones; Synergistic effect; Toxicity; Chlorpyrifos; Daidzein; Corticosterone; Genistein; Ovary histopathology
• ISSN: 2005-9752; 2233-7784
• DOI: 10.1007/s13530-022-00137-6

Objective Chlorpyrifos (CF) residues detected in crops because of its widespread use as insecticide. CF toxicity linked to the oxidative stress and lipid peroxides accumulation. Soy isoflavones, mostly genistein (GS) and daidzein (DZ) are antioxidants. The present research aim was to know the effects of either DZ, GS or their combinations with CF on the reproductive performance of female rats and to investigate whether GN or DZ might have protection against pesticide toxicity. Methods Healthy adult female albino rats divided into five groups of six rats each. The first group was control received corn oil, second group treated with CF 6.7 mg/kg, third group treated with CF 6.7 mg/kg + GS 21.7 mg/kg, forth group treated with CF 6.7 mg/kg + DZ 17.4 mg/kg and the last group given 6.7 mg/kg CF + 21.7 mg/kg GS + 17.4 mg/kg DZ, all are oral daily doses for six weeks. In the end, rats sacrificed and the blood samples collected for biochemical analysis and ovary tissues collected for histopathology analysis. Results No deaths or changes in the appearance of animals reported throughout the study period. There was a significant body weight increase in all groups except for CF group. Treatment groups of CF alone or with either GS, DZ or their combination for 42 days show a significant reduction of serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH), in addition to significant increase in serum corticosterone level in female rats. Histopathology results of rat ovaries consistent with the biochemical tests. Conclusions GS or DZ provides no protective effect against oxidative stress induced by CF. However, GS and DZ together have a synergistic effect producing same effect as oxidative stress induced by CF. further studies required to confirm this synergistic effect and reproductive toxicity in female rats.