Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form

• Published in: ISRN Chromatography Vol. 2012; no. 2012; pp. 1 - 8
• Main Authors: Mahadik, Kakasaheb R., Sathiyanarayanan, L., Rathore, Atul S., Nikam, Ajinkya R., More, Snehal J., Tandulwadkar, Suparna S.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2012; International Scholarly Research Network; Hindawi Limited
• Subjects: Analgesics; Chromatography; Drug dosages; Methods; Pain; Pharmaceutical industry; Pharmacy; Product development; Studies
• ISSN: 2090-8636; 2090-8636
• DOI: 10.5402/2012/252895

A simple, precise, and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous determination of paracetamol (PCM), chlorzoxazone (CHZ), and nimesulide (NIM) in pharmaceutical dosage form. The chromatographic separation was achieved on a Thermo Hypersil GOLD C18 column (250 × 4.6 mm i.d., 5 μm particle size). The mobile phase consisted of water : acetonitrile (55 : 45 v/v). The flow rate was set to 1.2 mL min−1 and UV detection was carried out at 275 nm. The retention time (tR) for PCM, CHZ, and NIM was found to be 2.69 ± 0.02, 4.61 ± 0.01, and 9.55 ± 0.02 min, respectively. The validation of the proposed method was carried out for linearity, precision, robustness, limit of detection, limit of quantitation, specificity, and accuracy. The linear dynamic ranges were 32.5–65.0 μg mL−1 for PCM, 37.5–75.0 μg mL−1 for CHZ, and 10.0–20.0 μg mL−1 for NIM. The developed method can be used for routine quality control analysis of titled drugs in pharmaceutical dosage form.