Analogs of Human Plasminogen That Are Labeled with Fluorescence Probes at the Catalytic Site of the Zymogen

• Published in: The Journal of biological chemistry Vol. 271; no. 2; pp. 1072 - 1080
• Main Authors: Bock, Paul E., Day, Duane E., Verhamme, Ingrid M.A., Bernardo, M. Margarida, Olson, Steven T., Shore, Joseph D.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 12.01.1996; American Society for Biochemistry and Molecular Biology
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.271.2.1072

Fluorescent analogs of the proteinase zymogen, plasminogen (Pg), which are specifically inactivated and labeled at the catalytic site have been prepared and characterized as probes of the mechanisms of Pg activation. The active site induced non-proteolytically in Pg by streptokinase (SK) was inactivated stoichiometrically with the thioester peptide chloromethyl ketone, Nα-[(acetylthio)acetyl]-(D-Phe)-Phe-Arg-CH2Cl; the thiol group generated subsequently on the incorporated inhibitor with NH2OH was quantitatively labeled with the fluorescence probe, 2-((4′-iodoacetamido)anilino)naphthalene-6-sulfonic acid; and the labeled Pg was separated from SK. Cleavage of labeled [Glu]Pg1 by urokinase-type plasminogen activator (uPA) was accompanied by a fluorescence enhancement (ΔFmax/Fo) of 2.0, and formation of 1% plasmin (Pm) activity. Comparison of labeled and native [Glu]Pg1 as uPA substrates showed that activation of labeled [Glu]Pg1 generated [Glu]Pm1 as the major product, while native [Glu]Pg1 was activated at a faster rate and produced [Lys]Pm1 because of concurrent proteolysis by plasmin. When a mixture of labeled and native Pg was activated, to include plasmin-feedback reactions, the zymogens were activated at equivalent rates. The lack of potential proteolytic activity of the Pg derivatives allowed their interactions with SK to be studied under equilibrium binding conditions. SK bound to labeled [Glu]Pg1 and [Lys]Pg1 with dissociation constants of 590 ± 110 and 11 ± 7 nM, and fluorescence enhancements of 3.1 ± 0.1 and 1.6 ± 0.1, respectively. Characterization of the interaction of SK with native [Glu]Pg1 by the use of labeled [Glu]Pg1 as a probe indicated a ∼6-fold higher affinity of SK for the native Pg zymogen compared to the labeled Pg analog. Saturating levels of ε-aminocaproic acid reduced the affinity of SK for labeled [Glu]Pg1 by ∼2-fold and lowered the fluorescence enhancement to 1.8 ± 0.1, whereas the affinity of SK for labeled [Lys]Pg1 was reduced by ∼98-fold with little effect on the enhancement. These results demonstrate that occupation of lysine binding sites modulates the affinity of SK for Pg and the changes in the environment of the catalytic site associated with SK-induced conformational activation. Together, these studies show that the labeled Pg derivatives behave as analogs of native Pg which report functionally significant changes in the environment of the catalytic site of the zymogen.