Rapid purification of two lipase isoenzymes from Candida rugosa

We describe a two-step method for the purification of two lipases (lipases A and B) from C. rugosa. The purification procedure includes Phenyl-Sepharose and Sephacryl HR 100 chromatographies. The enzymes obtained were pure according to criteria of specific activity and neutral sugar content.

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Bibliographic Details
Published inBiotechnology techniques Vol. 8; no. 1; pp. 21 - 26
Main Authors Rua, M.L, Ballesteros, A
Format Journal Article
LanguageEnglish
Published 1994
Subjects
Candida rugosa
chromatography
isozymes
purification
triacylglycerol lipase
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Summary:We describe a two-step method for the purification of two lipases (lipases A and B) from C. rugosa. The purification procedure includes Phenyl-Sepharose and Sephacryl HR 100 chromatographies. The enzymes obtained were pure according to criteria of specific activity and neutral sugar content.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00207628