MEASUREMENT OF PLATELET ANTIBODY BY CARBOXYFLUORESCEIN DIACETATE (C-FDA) METHOD

• Published in: Journal of the Japan Society of Blood Transfusion Vol. 33; no. 4; pp. 379 - 384
• Main Authors: Minami, Nobuyuki, Imai, Shigemi, Tominaga, Takeshi, Wada, Hideo, Tsuda, Masayuki, Ookubo, Itsuko, Deguchi, Katsumi, Shirakawa, Shigeru, Masada, Masayuki, Katayama, Naoyuki
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Transfusion Medicine and Cell Therapy 1987
• ISSN: 0546-1448; 1883-8383
• DOI: 10.3925/jjtc1958.33.379

The Carboxyfluorescein Diacetate (C-FDA) Thrombocytotoxity method, mixed passive haemoagglutination (MPHA) method and lymphocytotoxity (LCT) test for the detection of antiplatelet antibodies were carried out in 24 patients with idiopathic thrombocytopenic purpura (ITP) and 11 multiply transfused patients. C-FDA method was possitive in 11 out of 14 ITP patients with poor response of therapy, 2 out of 10 ITP patients with good response of therapy and 6 out of 11 multiply transfused patients and this result was similar to that of LCT test. While MPHA method was positive in 5 out of 14 ITP patients with poor response of therapy and in 5 out of 11 multiply transfused patients. Platelet associated immunogulobulin G (PAIgG) and immunoglobulin M (PAIgM) were high in all case of ITP patients with possitive C-FDA method and in many patients with positive MPHA or LCT method, but in some cases with negative MPHA or LCT method. Furthermore, C-FDA method reacted with anti-platelet antibody, anti-GPIb antibody and anti-GPIIb/IIIa antibody, but LCT method could not react with anti-GPIb or GPIIb/IIIa antibody. A case of leukemic patients with alloantiplatelet antibodies and a case of malignant lymphoma with auto-antiplatelet antibody were described. C-FDA method was more sensitive and rapid than MPHA technique, this method was adequate for detection of antiplatelet antibodies.