Vitrification of Two-Cell Rat Embryos Derived from Immature HypothyroidrdwRats byin VitroFertilization in Ethylene Glycol-Based Solutions
Two-cell embryos derived from immaturerdwrats byin vitrofertilization (IVF) were vitrified in ethylene glycol-based solutions. Embryos exposed to EFS20 before being vitrified in EFS40 exhibited improved viabilityin vitro.All embryos exposed to EFS20 for 1–3 min before vitrification in EFS40 were mor...
Saved in:
| Published in | Cryobiology Vol. 38; no. 2; pp. 160 - 164 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier Inc
01.03.1999
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Two-cell embryos derived from immaturerdwrats byin vitrofertilization (IVF) were vitrified in ethylene glycol-based solutions. Embryos exposed to EFS20 before being vitrified in EFS40 exhibited improved viabilityin vitro.All embryos exposed to EFS20 for 1–3 min before vitrification in EFS40 were morphologically normal. However, 2–3 min of exposure to EFS20 increased the number of embryos that developed beyond the four-cell stage. More embryos exposed to EFS20 for 2–3 min developed to morulae (63–64%) and blastocysts (34–38%) than those exposed for 1 min (35 and 10%, respectively). After transfer, 33% of embryos exposed to EFS20 for 3 min and vitrified in EFS40 developed to term compared to 29% of fresh embryos. Fifteen (47%) of live young were homozygousrdwand all of the others were heterozygous rats. The present study demonstrated that vitrification in EFS solution can be routinely used to cryopreserve rat two-cell IVF-embryos with no loss of viability. |
|---|---|
| AbstractList | Two-cell embryos derived from immaturerdwrats byin vitrofertilization (IVF) were vitrified in ethylene glycol-based solutions. Embryos exposed to EFS20 before being vitrified in EFS40 exhibited improved viabilityin vitro.All embryos exposed to EFS20 for 1–3 min before vitrification in EFS40 were morphologically normal. However, 2–3 min of exposure to EFS20 increased the number of embryos that developed beyond the four-cell stage. More embryos exposed to EFS20 for 2–3 min developed to morulae (63–64%) and blastocysts (34–38%) than those exposed for 1 min (35 and 10%, respectively). After transfer, 33% of embryos exposed to EFS20 for 3 min and vitrified in EFS40 developed to term compared to 29% of fresh embryos. Fifteen (47%) of live young were homozygousrdwand all of the others were heterozygous rats. The present study demonstrated that vitrification in EFS solution can be routinely used to cryopreserve rat two-cell IVF-embryos with no loss of viability. |
| Author | Sato, E. Jiang, J.Y. Umezu, M. |
| Author_xml | – sequence: 1 givenname: J.Y. surname: Jiang fullname: Jiang, J.Y. – sequence: 2 givenname: M. surname: Umezu fullname: Umezu, M. – sequence: 3 givenname: E. surname: Sato fullname: Sato, E. |
| BookMark | eNp1kMtKAzEUhoNUsK1uXecFpiaZW2eptTcoCFrdhkxygpHMpCTTlvENfGsz1K2bHAj_93HOP0Gj1rWA0D0lM0pI8SB972a0qqoZozm9QmNKKpKwtGIjNCaE0oSxjNygSQhfJAJlmo3Rz4fpvNFGis64FjuN92eXLMBa_Co6vGzqaA34Gbw5gcLauwZvm0Z0Rw940x9c99l7Z5RX55gPuO5NiwenW4HvjDXfF3H8XcaohRbw2vbS2eRJhGh8c_Y4JMItutbCBrj7m1P0vlruF5tk97LeLh53iYy3FYmSlOSMMFaIiuRZqeeFqotUylRlJStLooiQmuqMpEUk0vjmQtOc1VBDqbN0imYXr_QuBA-aH7xphO85JXwokg9F8qFIPhQZgfkFgLjVyYDnQRpoJSjjQXZcOfMf-gvydX80 |
| CitedBy_id | crossref_primary_10_1016_j_cryobiol_2014_01_011 crossref_primary_10_1095_biolreprod63_6_1637 crossref_primary_10_1095_biolreprod63_5_1294 crossref_primary_10_1016_S0093_691X_03_00039_6 crossref_primary_10_1262_jrd_50_675 crossref_primary_10_1262_jrd_2018_035 crossref_primary_10_5536_KJPS_2013_40_3_197 crossref_primary_10_1007_s11248_011_9492_z crossref_primary_10_1038_s41684_022_01053_5 crossref_primary_10_1016_S1701_2163_16_31041_6 crossref_primary_10_1016_S0093_691X_02_01227_X crossref_primary_10_1016_S1701_2163_16_31040_4 crossref_primary_10_1095_biolreprod_104_032839 crossref_primary_10_1095_biolreprod_108_072918 crossref_primary_10_1016_j_fertnstert_2007_12_008 |
| Cites_doi | 10.1006/cryo.1996.1994 10.1007/BF02761753 10.1538/expanim1978.43.2_247 10.1095/biolreprod47.6.1134 10.1016/0378-4320(96)01536-9 10.1538/expanim1978.37.1_21 10.1016/0093-691X(93)90346-7 10.1530/jrf.0.1000021 10.1538/expanim1978.40.4_511 10.1016/0093-691X(89)90251-3 10.1530/jrf.0.0980139 10.1538/expanim1978.42.2_211 10.1006/cryo.1996.0046 |
| ContentType | Journal Article |
| Copyright | 1999 Academic Press |
| Copyright_xml | – notice: 1999 Academic Press |
| DBID | AAYXX CITATION |
| DOI | 10.1006/cryo.1999.2151 |
| DatabaseName | CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1090-2392 |
| EndPage | 164 |
| ExternalDocumentID | 10_1006_cryo_1999_2151 S001122409992151X |
| GroupedDBID | --- --K --M -~X .GJ .~1 0R~ 186 1B1 1RT 1~. 1~5 29F 4.4 457 4G. 53G 5GY 5RE 5VS 6J9 7-5 71M 8P~ 9JM AABVA AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALCJ AALRI AAOAW AAQFI AAQXK AATLK AAXUO ABBQC ABFRF ABGRD ABGSF ABJNI ABKYH ABLVK ABMAC ABMZM ABRWV ABTAH ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACRLP ADBBV ADEZE ADFGL ADMUD ADQTV ADUVX AEBSH AEFWE AEHWI AEKER AENEX AEQOU AESVU AEXOQ AFFNX AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CAG CBWCG COF CS3 DM4 DOVZS DU5 EBS EFBJH EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-Q GBLVA HLV HVGLF HZ~ H~9 IHE J1W KOM LCYCR LG5 LW8 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 QYZTP R2- RIG ROL RPZ SAB SDF SDG SDP SES SEW SNL SPCBC SSA SSH SSU SSZ T5K TWZ UHS UNMZH WUQ XPP ZCG ZMT ZXP ZY4 ~02 ~G- ~KM AAHBH AAXKI AAYXX ADVLN AFJKZ AKRWK CITATION |
| ID | FETCH-LOGICAL-c1996-dc10520226a90547f86db63cc3d472770d0acf1f4036c19336c5af152bebe7f43 |
| IEDL.DBID | .~1 |
| ISSN | 0011-2240 |
| IngestDate | Thu Sep 26 18:10:58 EDT 2024 Fri Feb 23 02:30:12 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | immature hypothyroidrdwrats vitrification ethylene glycol in vitrofertilization two-cell embryos |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c1996-dc10520226a90547f86db63cc3d472770d0acf1f4036c19336c5af152bebe7f43 |
| PageCount | 5 |
| ParticipantIDs | crossref_primary_10_1006_cryo_1999_2151 elsevier_sciencedirect_doi_10_1006_cryo_1999_2151 |
| PublicationCentury | 1900 |
| PublicationDate | March 1999 1999-03-00 |
| PublicationDateYYYYMMDD | 1999-03-01 |
| PublicationDate_xml | – month: 03 year: 1999 text: March 1999 |
| PublicationDecade | 1990 |
| PublicationTitle | Cryobiology |
| PublicationYear | 1999 |
| Publisher | Elsevier Inc |
| Publisher_xml | – name: Elsevier Inc |
| References | Kasai (RF6) 1996; 42 Zhu, Kasai, Otoge, Sakurai, Machida (RF17) 1993; 98 Kasai (RF7) 1997; 7 Jiang, Miyoshi, Umezu, Sato (RF4) 1997; 42 Miyoshi, Funahashi, Okuda, Niwa (RF12) 1994; 100 Miyake, Kasai, Zhu, Sakurai, Machida (RF11) 1993; 40 Kasai, Zhu, Pedro, Nakamura, Sakurai, Edashige (RF9) 1996; 33 Cooke, Arambepola (RF2) 1997 Koto, Sato, Ikamoto, Adachi (RF10) 1988; 37 Anzai, Nakagata, Matsumoto, Takahashi, Takahash, Miyata (RF1) 1994; 43 Kasai, Nishimori, Zhu, Sakurai, Machida (RF8) 1992; 47 Tada, Sato, Mizorogi, Kasai, Ogawa (RF14) 1995; 45 Rall, Meyer (RF13) 1989; 31 Isachenko, Isachenko, Ostashko, Grishchenko (RF3) 1997; 34 Jiang, Umezu (RF5) 1997; 14 Umezu, Fujimura, Sugawara, Kagabu (RF15) 1993; 42 Umezu, Kawada, Ishii-Miwa, Ishii, Masaki (RF16) 1991; 40 Kasai (10.1006/cryo.1999.2151_RF8) 1992; 47 Rall (10.1006/cryo.1999.2151_RF13) 1989; 31 Isachenko (10.1006/cryo.1999.2151_RF3) 1997; 34 Kasai (10.1006/cryo.1999.2151_RF9) 1996; 33 Kasai (10.1006/cryo.1999.2151_RF7) 1997; 7 Umezu (10.1006/cryo.1999.2151_RF16) 1991; 40 Zhu (10.1006/cryo.1999.2151_RF17) 1993; 98 Miyake (10.1006/cryo.1999.2151_RF11) 1993; 40 Koto (10.1006/cryo.1999.2151_RF10) 1988; 37 Cooke (10.1006/cryo.1999.2151_RF2) 1997 Umezu (10.1006/cryo.1999.2151_RF15) 1993; 42 Tada (10.1006/cryo.1999.2151_RF14) 1995; 45 Jiang (10.1006/cryo.1999.2151_RF4) 1997; 42 Kasai (10.1006/cryo.1999.2151_RF6) 1996; 42 Miyoshi (10.1006/cryo.1999.2151_RF12) 1994; 100 Anzai (10.1006/cryo.1999.2151_RF1) 1994; 43 Jiang (10.1006/cryo.1999.2151_RF5) 1997; 14 |
| References_xml | – volume: 40 start-page: 511 year: 1991 end-page: 515 ident: RF16 article-title: Pituitary and plasma levels of growth hormone (GH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) in hereditary dwarf rats ( publication-title: Exp. Anim. contributor: fullname: Masaki – volume: 42 start-page: 211 year: 1993 end-page: 216 ident: RF15 article-title: Pituitary and serum levels of prolactin (PRL), thyroid stimulating hormone (TSH) and serum thyroxine (T4) in hereditary dwarf rats ( publication-title: Exp. Anim. contributor: fullname: Kagabu – volume: 40 start-page: 121 year: 1993 end-page: 134 ident: RF11 article-title: Vitrification of mouse oocytes and embryos at various stages of development in an ethylene glycol-based solution by a simple method publication-title: Theriogenology contributor: fullname: Machida – volume: 37 start-page: 21 year: 1988 end-page: 30 ident: RF10 publication-title: Exp. Anim. contributor: fullname: Adachi – volume: 45 start-page: 323 year: 1995 end-page: 325 ident: RF14 article-title: Efficient cryopreservation of hairless mutant (bald) and normal Wistar rat embryos by vitrification publication-title: Lab. Anim. Sci. contributor: fullname: Ogawa – volume: 31 start-page: 683 year: 1989 end-page: 692 ident: RF13 article-title: Zona fracture damage and its avoidance during the cryopreservation of mammalian embryos publication-title: Theriogenology contributor: fullname: Meyer – volume: 33 start-page: 459 year: 1996 end-page: 464 ident: RF9 article-title: Fracture damage of embryos and its prevention during vitrification and warming publication-title: Cryobiology contributor: fullname: Edashige – volume: 42 start-page: 67 year: 1996 end-page: 75 ident: RF6 article-title: Simple and efficient methods for vitrification of mammalian embryos publication-title: Anim. Reprod. Sci. contributor: fullname: Kasai – volume: 43 start-page: 247 year: 1994 end-page: 250 ident: RF1 article-title: Cryopreservation of in vitro fertilized embryos from transgenic rat by ultrarapid freezing publication-title: Exp. Anim. contributor: fullname: Miyata – volume: 100 start-page: 21 year: 1994 end-page: 26 ident: RF12 article-title: Development of rat one-cell embryos in a chemically defined medium: Effects of glucose, phosphate and osmolarity publication-title: J. Reprod. Fertil. contributor: fullname: Niwa – volume: 34 start-page: 157 year: 1997 end-page: 164 ident: RF3 article-title: Ultrarapid freezing of rat embryos with rapid dilution of permeable cryoprotectants publication-title: Cryobiology contributor: fullname: Grishchenko – volume: 42 start-page: 188 year: 1997 ident: RF4 article-title: In vitro fertilization and development of superovulated oocytes derived from immature publication-title: Jpn. J. Fertil. Steril. contributor: fullname: Sato – volume: 47 start-page: 1134 year: 1992 end-page: 1139 ident: RF8 article-title: Survival of mouse morulae vitrified in an ethylene glycol-based solution after exposure to the solution at various temperatures publication-title: Biol. Reprod. contributor: fullname: Machida – volume: 7 start-page: 173 year: 1997 end-page: 179 ident: RF7 article-title: Cryopreservation of mammalian embryos publication-title: Mol. Biotech. contributor: fullname: Kasai – volume: 98 start-page: 139 year: 1993 end-page: 145 ident: RF17 article-title: Cryopreservation of expanded mouse blastocysts by vitrification in ethylene glycol-based solutions publication-title: J. Reprod. Fertil. contributor: fullname: Machida – year: 1997 ident: RF2 article-title: Thyroid hormone effects on testicular growth, maturation and function publication-title: Current Advances in Andrology contributor: fullname: Arambepola – volume: 14 start-page: 86 year: 1997 ident: RF5 article-title: Hormone requirements in the induction of ovulation in immature publication-title: J. Mamm. Ova Res. contributor: fullname: Umezu – volume: 34 start-page: 157 year: 1997 ident: 10.1006/cryo.1999.2151_RF3 article-title: Ultrarapid freezing of rat embryos with rapid dilution of permeable cryoprotectants publication-title: Cryobiology doi: 10.1006/cryo.1996.1994 contributor: fullname: Isachenko – volume: 7 start-page: 173 year: 1997 ident: 10.1006/cryo.1999.2151_RF7 article-title: Cryopreservation of mammalian embryos publication-title: Mol. Biotech. doi: 10.1007/BF02761753 contributor: fullname: Kasai – volume: 45 start-page: 323 year: 1995 ident: 10.1006/cryo.1999.2151_RF14 article-title: Efficient cryopreservation of hairless mutant (bald) and normal Wistar rat embryos by vitrification publication-title: Lab. Anim. Sci. contributor: fullname: Tada – volume: 43 start-page: 247 year: 1994 ident: 10.1006/cryo.1999.2151_RF1 article-title: Cryopreservation of in vitro fertilized embryos from transgenic rat by ultrarapid freezing publication-title: Exp. Anim. doi: 10.1538/expanim1978.43.2_247 contributor: fullname: Anzai – year: 1997 ident: 10.1006/cryo.1999.2151_RF2 article-title: Thyroid hormone effects on testicular growth, maturation and function contributor: fullname: Cooke – volume: 47 start-page: 1134 year: 1992 ident: 10.1006/cryo.1999.2151_RF8 article-title: Survival of mouse morulae vitrified in an ethylene glycol-based solution after exposure to the solution at various temperatures publication-title: Biol. Reprod. doi: 10.1095/biolreprod47.6.1134 contributor: fullname: Kasai – volume: 42 start-page: 188 year: 1997 ident: 10.1006/cryo.1999.2151_RF4 article-title: In vitro fertilization and development of superovulated oocytes derived from immaturerdw publication-title: Jpn. J. Fertil. Steril. contributor: fullname: Jiang – volume: 42 start-page: 67 year: 1996 ident: 10.1006/cryo.1999.2151_RF6 article-title: Simple and efficient methods for vitrification of mammalian embryos publication-title: Anim. Reprod. Sci. doi: 10.1016/0378-4320(96)01536-9 contributor: fullname: Kasai – volume: 37 start-page: 21 year: 1988 ident: 10.1006/cryo.1999.2151_RF10 article-title: rdw publication-title: Exp. Anim. doi: 10.1538/expanim1978.37.1_21 contributor: fullname: Koto – volume: 40 start-page: 121 year: 1993 ident: 10.1006/cryo.1999.2151_RF11 article-title: Vitrification of mouse oocytes and embryos at various stages of development in an ethylene glycol-based solution by a simple method publication-title: Theriogenology doi: 10.1016/0093-691X(93)90346-7 contributor: fullname: Miyake – volume: 100 start-page: 21 year: 1994 ident: 10.1006/cryo.1999.2151_RF12 article-title: Development of rat one-cell embryos in a chemically defined medium: Effects of glucose, phosphate and osmolarity publication-title: J. Reprod. Fertil. doi: 10.1530/jrf.0.1000021 contributor: fullname: Miyoshi – volume: 40 start-page: 511 year: 1991 ident: 10.1006/cryo.1999.2151_RF16 article-title: Pituitary and plasma levels of growth hormone (GH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) in hereditary dwarf rats (rdwrdw publication-title: Exp. Anim. doi: 10.1538/expanim1978.40.4_511 contributor: fullname: Umezu – volume: 31 start-page: 683 year: 1989 ident: 10.1006/cryo.1999.2151_RF13 article-title: Zona fracture damage and its avoidance during the cryopreservation of mammalian embryos publication-title: Theriogenology doi: 10.1016/0093-691X(89)90251-3 contributor: fullname: Rall – volume: 98 start-page: 139 year: 1993 ident: 10.1006/cryo.1999.2151_RF17 article-title: Cryopreservation of expanded mouse blastocysts by vitrification in ethylene glycol-based solutions publication-title: J. Reprod. Fertil. doi: 10.1530/jrf.0.0980139 contributor: fullname: Zhu – volume: 42 start-page: 211 year: 1993 ident: 10.1006/cryo.1999.2151_RF15 article-title: Pituitary and serum levels of prolactin (PRL), thyroid stimulating hormone (TSH) and serum thyroxine (T4) in hereditary dwarf rats (rdw/rdw publication-title: Exp. Anim. doi: 10.1538/expanim1978.42.2_211 contributor: fullname: Umezu – volume: 33 start-page: 459 year: 1996 ident: 10.1006/cryo.1999.2151_RF9 article-title: Fracture damage of embryos and its prevention during vitrification and warming publication-title: Cryobiology doi: 10.1006/cryo.1996.0046 contributor: fullname: Kasai – volume: 14 start-page: 86 year: 1997 ident: 10.1006/cryo.1999.2151_RF5 article-title: Hormone requirements in the induction of ovulation in immaturerdw publication-title: J. Mamm. Ova Res. contributor: fullname: Jiang |
| SSID | ssj0006734 |
| Score | 1.5498757 |
| Snippet | Two-cell embryos derived from immaturerdwrats byin vitrofertilization (IVF) were vitrified in ethylene glycol-based solutions. Embryos exposed to EFS20 before... |
| SourceID | crossref elsevier |
| SourceType | Aggregation Database Publisher |
| StartPage | 160 |
| SubjectTerms | ethylene glycol immature hypothyroidrdwrats in vitrofertilization two-cell embryos vitrification |
| Title | Vitrification of Two-Cell Rat Embryos Derived from Immature HypothyroidrdwRats byin VitroFertilization in Ethylene Glycol-Based Solutions |
| URI | https://dx.doi.org/10.1006/cryo.1999.2151 |
| Volume | 38 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8NAEF5EEbyIT3yzB8FT7Ibsbpqj1taq6EFUegv7hEDblLQquXj3XzuTpD5AL15yWHaS8O0w32zyzSwhx7FLBFc8DqwRHjYoggVaMB5oZVUY63bEFG4Ub-9k_5FfD8RggXTmtTAoq2xifx3Tq2jdjLQaNFuTLMMa3xB_C2GKg7w1wAp2oD_w6dO3L5mHjKO6E3MYBjh73riRyZYpyhyr9ZJTtP-dmL6RTW-NrDZZIj2rX2SdLLjxBlmuz40sN8n7UzYrUONTwUpzTx9e86DjhkN6r2a0O9LwzCm9AO96cZZiCQm9Go2qHp60X05wdYo8s4V9hflTqstsTPGeeQ9l1sOmNpPCaBemAjE5ejkswWeCc2A9Sz8_pm2Rx173odMPmjMVAlPpja0JUfkCSZdKIFuLfVtaLSNjIsshlYmZZcr40HNgNrCI4CqUB5LXsNqx59E2WRznY7dDqGdKCiMTb2PFQ-7a3PBEe2EYc14mYpeczAFNJ3XrjLRukixThD5F6FOEfpeEc7zTH4ufQlz_w2bvHzb7ZKXuuoASsgOyOCue3SHkFDN9VDnNEVk6u7rp330A9RzOtA |
| link.rule.ids | 315,783,787,4509,24128,27936,27937,45597,45691 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3PT9swFLaACcEFsbGJbjB8mMQpraPYTnMcXbvy84Ba1Fvkn1KktqnSMpQLd_5r3kvSMSR24ZKDZTvR89P7PsffeybkR-wSwRWPA2uEhw2KYIEWjAdaWRXGuhsxhRvF6xs5HPOLiZhskN46FwZllU3sr2N6Fa2blk5jzc4iyzDHN8RjIaQ4iFuTTfKBIz8Gp24_vug8ZBzVpZjDMMDu68qNTHZMUeaYrpe0cYK3kekftBnsk72GJtKf9Zd8JBtu_ols1xdHlgfk6S5bFSjyqexKc09HD3nQc9MpvVUr2p9peOeS_gL3-uMsxRwSej6bVUU86bBc4PIUeWYL-wD9l1SX2ZzinPkAddbTJjmTQmsfugIyOfp7WoLTBGcAe5b-_Zv2mYwH_VFvGDSXKgSmEhxbE6L0BViXSoCuxb4rrZaRMZHlwGViZpkyPvQcoA1GRPAUygPKa1ju2PPoC9ma53N3SKhnSgojE29jxUPuutzwRHthGHNeJqJFTtcGTRd17Yy0rpIsUzR9iqZP0fQtEq7tnb5a_RQC-3_GfH3HmBOyMxxdX6VX5zeX38huXYIB9WRHZGtV3LtjIBgr_b1yoGfz_tBN |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Vitrification+of+Two-Cell+Rat+Embryos+Derived+from+Immature+HypothyroidrdwRats+byin+VitroFertilization+in+Ethylene+Glycol-Based+Solutions&rft.jtitle=Cryobiology&rft.au=Jiang%2C+J.Y.&rft.au=Umezu%2C+M.&rft.au=Sato%2C+E.&rft.date=1999-03-01&rft.issn=0011-2240&rft.volume=38&rft.issue=2&rft.spage=160&rft.epage=164&rft_id=info:doi/10.1006%2Fcryo.1999.2151&rft.externalDBID=n%2Fa&rft.externalDocID=10_1006_cryo_1999_2151 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0011-2240&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0011-2240&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0011-2240&client=summon |