Vitrification of Two-Cell Rat Embryos Derived from Immature HypothyroidrdwRats byin VitroFertilization in Ethylene Glycol-Based Solutions

Two-cell embryos derived from immaturerdwrats byin vitrofertilization (IVF) were vitrified in ethylene glycol-based solutions. Embryos exposed to EFS20 before being vitrified in EFS40 exhibited improved viabilityin vitro.All embryos exposed to EFS20 for 1–3 min before vitrification in EFS40 were mor...

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Published inCryobiology Vol. 38; no. 2; pp. 160 - 164
Main Authors Jiang, J.Y., Umezu, M., Sato, E.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.03.1999
Subjects
ethylene glycol
immature hypothyroidrdwrats
in vitrofertilization
two-cell embryos
vitrification
immature hypothyroidrdwrats
vitrification
ethylene glycol
in vitrofertilization
two-cell embryos
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Summary:Two-cell embryos derived from immaturerdwrats byin vitrofertilization (IVF) were vitrified in ethylene glycol-based solutions. Embryos exposed to EFS20 before being vitrified in EFS40 exhibited improved viabilityin vitro.All embryos exposed to EFS20 for 1–3 min before vitrification in EFS40 were morphologically normal. However, 2–3 min of exposure to EFS20 increased the number of embryos that developed beyond the four-cell stage. More embryos exposed to EFS20 for 2–3 min developed to morulae (63–64%) and blastocysts (34–38%) than those exposed for 1 min (35 and 10%, respectively). After transfer, 33% of embryos exposed to EFS20 for 3 min and vitrified in EFS40 developed to term compared to 29% of fresh embryos. Fifteen (47%) of live young were homozygousrdwand all of the others were heterozygous rats. The present study demonstrated that vitrification in EFS solution can be routinely used to cryopreserve rat two-cell IVF-embryos with no loss of viability.
ISSN:0011-2240
1090-2392
DOI:10.1006/cryo.1999.2151