THE EFFECTS OF FILTRATION PROCEDURES AND BLOOD STORAGE PERIOD ON THE EFFICIENCIES OF POLYESTER FILTERS FOR PREPARING LEUKOCYTE REDUCED RED CELLS

• Published in: Journal of the Japan Society of Blood Transfusion Vol. 40; no. 4; pp. 655 - 660
• Main Authors: Yamamura, Hajime, Miyahara, Masayuki, Satoh, Hiromasa
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Transfusion Medicine and Cell Therapy 1994
• ISSN: 0546-1448; 1883-8383
• DOI: 10.3925/jjtc1958.40.655

To find the optimal protocol for preparing leukocyte-reduced red cells (LRRC) with polyester filters, such as Sepacell R-500N and Pall BPF4, we studied the effects of filtration procedures and blood storage period on their efficiencies. Pre-filtration dilution had no effect on leukocyte removal, but caused about 5% increase in red cell recovery and almost 50% reduction in filtration time when Pall BPF4 was used. On the other hand, post-filtration rinse resulted in a decrease in leukocyte removal by about 10 times and an increase in red cell recovery by about 5%. These procedures had the almost same effects on the filtration efficiencies of Sepacell R-500N. There were no significant differences in leukocyte removal and red cell recovery between two groups of starting red cells stored for 2-10 days or 42 days in a mannitol-adenine-saline (MAP) solution. However, 21 day-old cells in CPD showed higher hemolysis after filtration than 2 to 14 day-old red cells in CPD. These data indicate that pre-dilution is required but post-rinse should be avoided for achieving the maximum efficiencies of the polyester filters. The storage limit of red cells as a starting material for LRRC can be extended up to 14 days in CPD or 42 days in MAP. Thus, the use of the optimal protocol for Pall BPF4 led to 4 log of leukocyte reduction, 92% of red cell recovery and 11min of filtration time.