Leptin promotes tendon stem/progenitor cell senescence through the AKT-mTOR signaling pathway

• Published in: Experimental cell research Vol. 442; no. 2; p. 114274
• Main Authors: Lei, Changbin, Li, Yanmei, Chen, Jiafeng, Nie, Daibang, Song, Xin, Lei, Cece, Zhou, Yiqin, Wang, Wang, Sun, Jiuyi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2024
• Subjects: Leptin; mTOR; Senescence; Tendon; TSPC; mTOR; Senescence; TSPC; Leptin; Tendon; senescence; tendon; leptin
• ISSN: 0014-4827; 1090-2422; 1090-2422
• DOI: 10.1016/j.yexcr.2024.114274

Dysregulated adipokine production is an influencing factor for the homeostatic imbalance of tendons. High levels of serum leptin may be a potential link between increasing adiposity and tendinopathy, while the detailed mechanistic explanation was not well-defined. In this study, we investigated the regulatory role of leptin in the tendon stem/progenitor cells (TSPCs) and the molecular mechanism within, and determined the effect of high levels of leptin on tendon recovery. We demonstrated that leptin reduced the viability of isolated rat TSPCs in a dose-dependent way, accompanied with increased transdifferentiation and altered gene expression of a series of extracellular matrix (ECM) enzymatic modulators. Also, we found that leptin could dose-dependently promote TSPCs senescence, while exhibiting limited effect in apoptotic or autophagic induction. Mechanistic study evidenced that leptin treatment increased the AKT/mTOR signaling activity and elevated the expression of leptin receptor (LEPR) in TSPCs, without marked change in MAPK or STAT5 activation. Further, we confirmed that rapamycin treatment, but not AKT inhibition, effectively reduced the leptin-promoted TSPCs senescence. In a rat model with Achilles wounding, exposure to leptin profoundly delayed tendon healing, which was effectively rescued with rapamycin treatment. Our results suggested that leptin could cause intrinsic cellular deficits in TSPCs and impede tendon repair through the AKT/mTOR signaling pathway. These findings evidenced for an important role of elevated leptin levels in the care of tendinopathy and tendon tears. [Display omitted] •Leptin impairs the stemness of TSPCs.•Leptin drives TSPCs senescence via overactivation of AKT-mTOR signaling.•Leptin impedes Achilles tendon wound repair.•Rapamycin effectively rescues leptin-induced tendinopathic changes.