Decrease in the Linking Number of Plasmid DNA indnaAMutants ofEscherichia coli

We made use of agarose gel electrophoresis in the presence of chloroquine to examine the linking number of plasmids in temperature-sensitivednaAmutants, includingdnaA5anddnaA46mutants. The linking number of DNA prepared fromdnaAmutants growing at 37°C was lower than that from wild type cells yet the...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 218; no. 1; pp. 137 - 141
Main Authors Mizushima, Tohru, Tanino, Junko, Miki, Takeyoshi, Sekimizu, Kazuhisa
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.01.1996
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Summary:We made use of agarose gel electrophoresis in the presence of chloroquine to examine the linking number of plasmids in temperature-sensitivednaAmutants, includingdnaA5anddnaA46mutants. The linking number of DNA prepared fromdnaAmutants growing at 37°C was lower than that from wild type cells yet there was no significant difference when cells were grown at 28°C. Complementation analysis with a plasmid containing the wild typednaAgene and phage P1-mediated transduction confirmed that mutations in thednaAgene were responsible for the decrease in the linking number of DNA.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0024