Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Gezielte Mutationen der Oberflächenschleife von Halogenalkan‐Dehalogenasen haben Auswirkungen auf die Enantiodiskriminierung. Die Temperaturabhängigkeit der Enantioselektivität (siehe lnE‐1/T‐Kurve) von β‐Bromalkanen kehrt sich bei einer Deletion der Oberflächenschleife um (rote Datenpunkte), was be...

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Published inAngewandte Chemie Vol. 122; no. 35; pp. 6247 - 6251
Main Authors Prokop, Zbynek, Sato, Yukari, Brezovsky, Jan, Mozga, Tomas, Chaloupkova, Radka, Koudelakova, Tana, Jerabek, Petr, Stepankova, Veronika, Natsume, Ryo, van Leeuwen, Jan G. E., Janssen, Dick B., Florian, Jan, Nagata, Yuji, Senda, Toshiya, Damborsky, Jiri
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 16.08.2010
WILEY‐VCH Verlag
Subjects
Enantioselektivität
Enzyme
Halogenalkan-Dehalogenasen
Protein-Engineering
Thermodynamik
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Summary:Gezielte Mutationen der Oberflächenschleife von Halogenalkan‐Dehalogenasen haben Auswirkungen auf die Enantiodiskriminierung. Die Temperaturabhängigkeit der Enantioselektivität (siehe lnE‐1/T‐Kurve) von β‐Bromalkanen kehrt sich bei einer Deletion der Oberflächenschleife um (rote Datenpunkte), was bei Einführung einer zusätzlichen Einzelpunktmutation wieder rückgängig gemacht wird; bei α‐Bromestern findet keine Änderung statt.
Bibliography:ArticleID:ANGE201001753
Grants-in-Aid from Ministry of Education, Culture, Sports, Science, and Technology, Japan and the Ministry of Agriculture, Forestry, and Fisheries
EMBO
Ministry of Education, Youth, and Sports of the Czech Republic - No. LC06010; No. MSM0021622412
ark:/67375/WNG-TFTX7P3X-0
Z.P. acknowledges EMBO for financial support of his stay at the University of Groningen. Financial support is gratefully acknowledged from: the Ministry of Education, Youth, and Sports of the Czech Republic (grants LC06010 to J.D. and MSM0021622412 to Z.P.); the Grant Agency of the Czech Academy of Sciences (grant no. IAA401630901 to J.B.); the Grants-in-Aid from Ministry of Education, Culture, Sports, Science, and Technology, Japan and the Ministry of Agriculture, Forestry, and Fisheries, Japan (Y.N.); and the New Energy and Industrial Technology Development Organization (NEDO) of Japan (T.S.). Access to the METACentrum supercomputing facilities is highly appreciated (MSM6383917201). We thank Prof. Uwe Bornscheuer from the University of Greifswald for critical reading of this manuscript.
Czech Academy of Sciences - No. IAA401630901
istex:7397A451A60BE37C51DE18CCCAD3814A70640CAC
New Energy and Industrial Technology Development Organization (NEDO) of Japan
Z.P. acknowledges EMBO for financial support of his stay at the University of Groningen. Financial support is gratefully acknowledged from: the Ministry of Education, Youth, and Sports of the Czech Republic (grants LC06010 to J.D. and MSM0021622412 to Z.P.); the Grant Agency of the Czech Academy of Sciences (grant no. IAA401630901 to J.B.); the Grants‐in‐Aid from Ministry of Education, Culture, Sports, Science, and Technology, Japan and the Ministry of Agriculture, Forestry, and Fisheries, Japan (Y.N.); and the New Energy and Industrial Technology Development Organization (NEDO) of Japan (T.S.). Access to the METACentrum supercomputing facilities is highly appreciated (MSM6383917201). We thank Prof. Uwe Bornscheuer from the University of Greifswald for critical reading of this manuscript.
These authors contributed equally to this work.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201001753