FEASIBILITY OF CD34-POSITIVE SELECTION USING THE AVIDIN-BIOTIN IMMUNOADSORPTION SYSTEM

• Published in: Journal of the Japan Society of Blood Transfusion Vol. 45; no. 5; pp. 611 - 616
• Main Authors: Tajima, Naoko, Yano, Shingo, Asai, Osamu, Yahagi, Yuichi, Kuraishi, Yasunobu, Watanabe, Hiroshi, Kato, Akinori, Hoshi, Yasutaka, Katori, Mituji, Maki, Nobuko, Nagamine, Mamoru, Kobayashi, Tadashi
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Transfusion Medicine and Cell Therapy 1999
• Subjects: CD34 positive selection; minimal residual disease
• ISSN: 0546-1448; 1883-8383
• DOI: 10.3925/jjtc1958.45.611

To investigate the feasibility of CD34-positive selection, we separated CD34+ cells from KGla cells, fresh bone marrow samples (obtained from 2 ALL patients, 1 NHL, 1 breast cancer and 4 healthy volunteers) and frozen samples (3 ALL) by the avidin-biotin immunoadsorption using a CEPRATE LC KITS® (CellPro Inc.). We then detected minimal residual disease (MRD) from a separated Ph-positive ALL fresh sample by the PCR method. The yield of selected CD34+ cells were 78.9% (KGla cells), 45.5% (fresh bone marrow sample obtained from ALL, NHL and breast cancer), 9.3% (healthy volunteers) and 3.9% (frozen sample obtained from ALL). A sample of Ph ALL was able to eliminate MRD from the CD34+ fraction. We conclude that CD34-positive selection by the avidinbiotin immunoadsorption is useful in fresh samples but not from frozen samples and we demonstrated that this procedure may deplete tumor cells in a patient with ALL.