Immunofluorescence Analysis of Estrogen Receptors Alpha Expression in Non-Small Cell Lung Cancer Tissue

• Published in: Moscow University chemistry bulletin Vol. 79; no. 3; pp. 182 - 188
• Main Authors: Bogush, T. A., Basharina, A. A., Romanov, I. P., Grishanina, A. N., Bogush, E. A., Scherbakov, A. M., Ravcheeva, A. B., Lee, A., Kosorukov, V. S.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.06.2024; Springer Nature B.V
• Subjects: Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Death; Estrogens; Flow cytometry; Immunofluorescence; Low level; Original Article; Receptors; non-small cell lung cancer; immunofluorescence analysis; flow cytometry; prognosis; Kaplan-Meier method; estrogen receptor alpha
• ISSN: 0027-1314; 1935-0260
• DOI: 10.3103/S0027131424700159

A quantitative assessment of the expression of estrogen receptors alpha (ERα) is carried out in 115 samples of non-small cell lung cancer (NSCLC) by an immunofluorescent assay and flow cytometry. It is shown that a high level of ERα of ≥20% predicts a higher aggressiveness of NSCLC than at a low level of <20%: median survival based on a follow-up period of 78 months increases by 1.5 times; the risk of death is reduced by a factor of almost 2.0 ( p = 0.04). The time to death increases on average by 18 months in about 20% of patients with a low ERα expression. The results validate the informative value of the immunofluorescence analysis and flow cytometry for quantifying the ERα expression and substantiate the prospects of antiestrogen therapy as a new option for NSCLC treatment, in particular, by analogy with breast cancer—in a long-term adjuvant therapy in ERα + NSCLC patients.