The Role of Components of the Extracellular Matrix in the Differentiation of Human Pluripotent Cells into Ectodermal Derivatives

• Published in: Russian journal of developmental biology Vol. 54; no. 1; pp. 37 - 53
• Main Authors: Ognivtsev, A. A., Kalabusheva, E. P., Osidak, E. O., Domogatsky, S. P., Vorotelyak, E. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.02.2023; Springer Nature B.V
• Subjects: Animal Anatomy; Biomedical and Life Sciences; Cell differentiation; Collagen (type I); Developmental Biology; Ectoderm; Embryogenesis; Extracellular matrix; Growth factors; Histology; Laminin; Life Sciences; Morphology; Neuroectoderm; Neuronal-glial interactions; Original Research; Pluripotency; Stem cells; Yes-associated protein; epidermal differentiation; YAP1; collagen; laminin; neural differentiation; iPSC
• ISSN: 1062-3604; 1608-3326
• DOI: 10.1134/S1062360423010101

One of the most important events in the embryonic development of mammals is the division of the ectoderm into integumentary and neuroectoderm. Signaling cascades induced by growth factors and cytokines involved in these processes have been studied in detail in recent decades. At the same time, the contribution of extracellular matrix (ECM) to these differentiation lineages remains unknown for mammals, while the significance of ECM in this process has been shown for other model organisms. To assess the effect of ECM on the formation of ectodermal derivatives, we modeled the neural and epidermal differentiation of human induced pluripotent stem cells (iPSCs) using substrates consisting of various ECM molecules and also studied the involvement of one of the central links of the ECM signaling cascades, a transcriptional coactivator YAP1 in differentiation processes. Our results revealed the stimulatory effect of laminin 332 on the early stages of epidermal differentiation and of type I and III collagens on the induction of the glial fate of late neural differentiation.