Alterations in Hemostatic and Hematological Parameters after Gastric Ulcer Induction in Rats. Possible Role of IL-6 and TNF-α

• Published in: Journal of evolutionary biochemistry and physiology Vol. 59; no. 1; pp. 82 - 93
• Main Authors: Saad, Ramadan A., Qutob, Haitham MH
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 2023; Springer Nature B.V
• Subjects: Acetic acid; Animal models; Animal Physiology; Biochemistry; Biomedical and Life Sciences; Evolutionary Biology; Experimental Papers; Fibrinogen; Gastric mucosa; Hematology; Inflammation; Interleukin 6; Life Sciences; Lipid peroxidation; Oxidative stress; Peptic ulcers; Prothrombin; Thrombin; Thromboplastin; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Tumors; Ulcers; inflammatory markers; peptic ulcer; oxidative stress; hematological parameters; hemostatic parameters
• ISSN: 0022-0930; 1608-3202
• DOI: 10.1134/S0022093023010076

This study examined the changes in hematological and hemostatic parameters in response to gastric mucosal injury in male Wistar rats divided into two major groups: acute and chronic. Each group was further subdivided into two subgroups: acute control (C1) and acute ulcer (U1), and chronic control (C2) and chronic ulcer (U2). Two experimental models were used in this study for peptic ulcer induction; the acute ulcer model was induced by pyloric ligation, and chronic gastric ulcers were induced by sub-serosal application of concentrated acetic acid. Gastric contents were collected for volume and pH determination in addition to macroscopic quantification of the degree of ulceration. Blood samples were examined for hemostatic parameters (prothrombin time; PT, activated partial thromboplastin time; aPTT) and other quantitative hemostatic parameters (fibrinogen, anti-thrombin III, D-dimer). Complete blood count and biochemical serum analysis of interleukin 6 (IL-6) and tumour necrosis factor α (TNF-α) were also performed. The results revealed an increase in serum IL-6 and TNF-α in both acute and chronic ulcer models in addition to elevated fibrinogen, especially in the chronic model. D-dimer was also significantly increased in the chronic model. Positive correlation is shown between inflammatory cytokines and fibrinogen and D-dimer. An increase of oxidative stress markers was also found in the stomach tissue of rats after induction of ulcers. The hemostatic and hematological changes might be due to elevated circulating inflammatory markers during ulceration via a lipid peroxidation-dependent mechanism, especially in the chronic state.