Characteristics of Actin—Myosin Interaction in Different Regions of Rat Heart

The ventricles and atria of the mammalian heart have differences in structure and function at different levels of organization. However, the current data on mechanical function of the heart chambers at different levels are contradictory and demand a detailed study. We compared mechanical characteris...

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Bibliographic Details
Published inJournal of evolutionary biochemistry and physiology Vol. 58; no. Suppl 1; pp. S98 - S106
Main Authors Gerzen, O. P., Votinova, V. O., Potoskueva, Iu. K., Nabiev, S. R., Nikitina, L. V.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.12.2022
Springer Nature B.V
Subjects
Actin
Animal Physiology
Biochemistry
Biomedical and Life Sciences
Evolutionary Biology
Experimental Papers
Filaments
Gel electrophoresis
Heart
Life Sciences
Mechanical properties
Muscle contraction
Myocardium
Myosin
Polyacrylamide
Regulatory proteins
Sodium lauryl sulfate
Structure-function relationships
Velocity
Ventricle
cardiac myosin
native thin filament
in vitro motility assay
myocardium region
actin–myosin interaction
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Summary:The ventricles and atria of the mammalian heart have differences in structure and function at different levels of organization. However, the current data on mechanical function of the heart chambers at different levels are contradictory and demand a detailed study. We compared mechanical characteristics of actin–myosin interaction by an in vitro motility assay and structural characteristics of contractile and regulatory proteins by one-dimensional denaturating polyacrylamide gel electrophoresis (SDS-PAGE) in atria, septum, right, and left ventricles. The sliding velocity of the reconstituted thin filaments over atrial myosin was significantly higher compared to other myocardium regions. No differences were observed in sliding velocity of atrial, septal, and ventricular native thin filaments over the myosin from the same myocardium region of the same rats. At the same time, the sliding velocity of the atrial native thin filament over porcine myosin was lower than that of the ventricular and septal native thin filament.
ISSN:0022-0930
1608-3202
DOI:10.1134/S0022093022070110