Effects of Lipopolysaccharide from Periodontopathic Bacteria and Interleukin-1 on Fibronectin Synthesis in Cultured Human Fibroblasts

• Published in: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 34; no. 3; pp. 654 - 664
• Main Authors: IZUMI, Yuichi, TANIGUCHI, Takuro, ONO, Tomonori, SETOGUCHI, Takashi, SUEDA, Takeshi, KUBO, Kohji, MATSUYAMA, Takashi
• Format: Journal Article
• Language: Japanese
• Published: JAPANESE SOCIETY OF PERIODONTOLOGY 1992
• Subjects: Fibroblasts; Fibronectin; IL-1; LPS
• ISSN: 0385-0110; 1880-408X
• DOI: 10.2329/perio.34.3_654

We studied the effects of lipopolysaccharide LPS) from Porphyromonas gingivalis, Prevotella intermedia and Porphyromonas gingivalis wild type, and interleukin-1 (IL-1) on production of fibronectin in cultured human fibroblasts. Fibroblasts were obtained from healthy gingiva, inflamed gingiva and healthy periodontal ligament. After the cells had reached confluence, they were further incubated with or without LPS (final concentration: 10. μg/ml) and/or IL-1 (final concentraion: 0.5-5. μg/ml) for up to 48 hours. Fibronectin levels in culture media were measured using enzyme linked immunosorbent assay. Fibronectin levels were increased by in fibroblasts stimulation with LPS or IL-1. Fibronectin levels stimulated by LPS+IL-1 were much increased compared to those stimulated by LPS or IL-1 alone. From these findings, it is suggested that LPS and/or IL-1 may promote fibronectin synthesis in fibroblasts of periodontal tissue in vivo.