Optimisation of Candida rugosa Lipase Esterase Activity

• Published in: Biotechnology (Faisalābād, Pakistan) Vol. 7; no. 1; pp. 112 - 117
• Main Authors: Korbekandi, H., Abedi, D., Pourhossein, M., Motovali-Bashi, M., Hejazi, M., Narimousaei, M., Kabiri, M.
• Format: Journal Article
• Language: English
• Published: 2008
• Subjects: Candida rugosa
• ISSN: 1682-296X; 1682-2978
• DOI: 10.3923/biotech.2008.112.117

We aimed to optimize esterase production of Candida rugosa lipase (CRL). Active culture of C. rugosa (DSM 2031) was revived and the culture medium containing the most frequently used ingredients was optimized using a fraction of factorial design method, Taguchi. Temperature and pH of the culture was also optimized using one factor at a time method. The optimum combination of the major medium ingredients, in order of their magnitude, was (g L super(-1)): Corn Steep Liquor (CSL) powder, (40), triolein (glyceril trioleate) (10), glucose (0) and oleic acid (2). The optimum temperature and pH were 30 degree C and 7, appropriately. Using this combination and conditions, esterase activity of the enzyme preparation was increased up to 9 U mL super(-1), which was equivalent to 20611 U mL super(-1) of Sigma registered lipase lipolytic activity.