Studies of the growth of human bone-derived cells in culture using aqueous two-phase partition

Human bone cells, maintained in culture, have been subjected to partitioning in an aqueous two-phase system on a countercurrent distribution apparatus. A broad cell distribution was obtained indicating cell-surface heterogeneity. Two major cell populations were identified which appeared to be growin...

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Bibliographic Details
Published inBioscience reports Vol. 4; no. 5; p. 415
Main Authors Sharpe, P T, MacDonald, B R, Gallagher, J A, Treffry, T E, Russell, R G
Format Journal Article
LanguageEnglish
Published England 01.05.1984
Subjects
Bone and Bones - cytology
Cell Differentiation
Cell Separation - methods
Cells, Cultured
Countercurrent Distribution
Dextrans
Humans
Osteoblasts - physiology
Polyethylene Glycols
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Summary:Human bone cells, maintained in culture, have been subjected to partitioning in an aqueous two-phase system on a countercurrent distribution apparatus. A broad cell distribution was obtained indicating cell-surface heterogeneity. Two major cell populations were identified which appeared to be growing at different rates. The 'fast'-growing cells had a less hydrophobic cell surface than the 'slow'-growing cells. Possible relationships of these cell populations with osteoblast differentiation and the potential importance of this technique in studies of osteoblast differentiation are discussed.
ISSN:0144-8463
DOI:10.1007/BF01122506