Age-, sex-, and strain-dependent differences in the induction of enzyme-altered islands in rat liver by diethylnitrosamine

The formation of foci with loss of ATPase and emergence of gamma-GTase was studied histochemically in livers of male and female Wistar and Sprague-Dawley rats of 3--4 and 6--7 weeks of age, respectively, after application of diethylnitrosamine. A single dose of 8 mg/kg body weight induced a consider...

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Bibliographic Details
Published inJournal of cancer research and clinical oncology Vol. 100; no. 2; p. 125
Main Authors Deml, E, Oesterle, D, Wolff, T, Greim, H
Format Journal Article
LanguageEnglish
Published Germany 01.06.1981
Subjects
Adenosine Triphosphatases - deficiency
Age Factors
Animals
Cytochrome P-450 Enzyme System - analysis
Diethylnitrosamine - toxicity
Female
gamma-Glutamyltransferase - analysis
Liver - drug effects
Liver - enzymology
Liver Neoplasms - chemically induced
Male
Nitrosamines - toxicity
Rats
Sex Factors
Species Specificity
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Summary:The formation of foci with loss of ATPase and emergence of gamma-GTase was studied histochemically in livers of male and female Wistar and Sprague-Dawley rats of 3--4 and 6--7 weeks of age, respectively, after application of diethylnitrosamine. A single dose of 8 mg/kg body weight induced a considerable island formation in weanlings of both sexes. Island induction in adults was observed only after repeated application. No difference in island size and number was observed with the exception of greater island size and Sprague-Dawley females. Sex-dependent differences in susceptibility to island induction were observed in weanlings, females being more sensitivity than males and Sprague-Dawley females being the most sensitive of all. No correlation was seen between monooxygenase activity and the extent of island formation. The coincidence of ATPase-deficiency and emergence of gamma-GTase was highest in Sprague-Dawley females. The importance of this result in respect to cancer formation is discussed. Weanling Sprague-Dawley females seemed to be the most suitable for use in a screening test system for chemical carcinogenicity, especially for testing low doses or weak carcinogens.
ISSN:0171-5216
DOI:10.1007/BF00403362