Electrochemical acetylcholinesterase biosensor for detection of cholinesterase inhibitors: study with eserine
Cholinesterase inhibitors are widely used as pesticides, as chemical warfare agents and as drugs to treat symptoms of Alzheimer’s disease. Therefore, it is a high need to develop methods for their detection which are fast, sensitive, and reliable. This paper reports a preliminary work in the develop...
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Published in | Informacije MIDEM (Spletna izd.) pp. 235 - 240 |
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Format | Journal Article |
Language | English |
Published |
01.02.2019
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Online Access | Get full text |
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Summary: | Cholinesterase inhibitors are widely used as pesticides, as chemical warfare agents and as drugs to treat symptoms of
Alzheimer’s disease. Therefore, it is a high need to develop methods for their detection which are fast, sensitive, and reliable. This
paper reports a preliminary work in the development of an electrochemical biosensor based on acetylcholinesterase (AChE) which is
constructed by immobilization layers – cysteamine/glutaraldehyde/AChE on thin layer gold electrode for detection of cholinesterase
inhibitors. Eserine (physostigmine) was used as a test inhibitor. The enzyme immobilization efficacy was evaluated by measuring
activity of immobilized enzyme via Ellman’s method. The enzyme activity of the initial reduction of 33% in five days remained after
that stable for at least one week. Chronoamperometric response to substrate acetylthiocholine chloride (ATCl) was assumed to follow
Michaelis-Menten kinetics. After exposure biosensor to 25 mM eserine for 10 min, 70% inhibition of enzyme was detected. Reactivation
factor of inhibited AChE was determined as 0.016 min-1. |
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ISSN: | 0352-9045 2232-6979 |
DOI: | 10.33180/InfMIDEM2018.406 |