Human interleukin-2 (IL-2) expressed by transfected mammalian cells

cDNA for human interleukin-2 (IL-2) was cloned into the pRc/RSV vector for expression in animal cells. Baby hamster kidney (BHK-21) cells and Chinese hamster ovary (CHO) cells were transfected several times using calcium phosphate and electroporation methods with the construct pRc/RSV SIGIL2. Differ...

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Published inPflügers Archiv Vol. 431; no. 6 Suppl 2; p. R227
Main Authors Knezević, M, Vorauer-Uhl, K, Hohenwarter, O, Steindl, F, Grabherr, R, Raspor, P
Format Journal Article
LanguageEnglish
Published Germany 1996
Subjects
Animals
Biological Assay
Cell Division - drug effects
Cell Line
CHO Cells
Cricetinae
Deoxyribonuclease HindIII
Escherichia coli - genetics
Escherichia coli - metabolism
Humans
Interleukin-2 - biosynthesis
Interleukin-2 - genetics
Interleukin-2 - pharmacology
Recombinant Proteins - biosynthesis
T-Lymphocytes - metabolism
Transfection
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Summary:cDNA for human interleukin-2 (IL-2) was cloned into the pRc/RSV vector for expression in animal cells. Baby hamster kidney (BHK-21) cells and Chinese hamster ovary (CHO) cells were transfected several times using calcium phosphate and electroporation methods with the construct pRc/RSV SIGIL2. Different transfection efficiencies were obtained. The biological test on CTLL-2 (mouse cytotoxic lymphocytes) showed that the kinetics of cell proliferation were different from those of rIL-2 (recombinant IL-2) expressed in bacteria and in BHK cells. When high concentrations of rIL-2 were applied, an inhibitory effect on CTLL-2 was observed when bacterial product was used, whilst rBHK interleukin caused no inhibition. Recombinant BHK IL2 induced a slower response of CTLL-2 cells at the beginning of the cultivation, however, prolonged activity was detected at the later stage of the experiment.
ISSN:0031-6768
DOI:10.1007/bf02346351