Screening, Isolation and Characterization of a Novel Antimicrobial Producing Actinomycete, Strain RAF10

A novel actinomycete strain designated RAF 10, producing antimicrobial substances was isolated from an Egyptian soil. Morphological and chemical studies indicated that strain RAF10 belonged to the genus Streptomyces. The comparison of its physiological characteristics with those of known species of...

Full description

Saved in:
Bibliographic Details
Published inBiotechnology (Faisalābād, Pakistan) Vol. 6; no. 4; pp. 489 - 496
Main Authors Rabah, Forar Laidi, Elshafei, Ali, Saker, Mahmoud, Cheikh, Bengraa, Hocine, Hacene
Format Journal Article
LanguageEnglish
Published 01.12.2007
Subjects
Streptomyces
Online AccessGet full text

Cover

More Information
Summary:A novel actinomycete strain designated RAF 10, producing antimicrobial substances was isolated from an Egyptian soil. Morphological and chemical studies indicated that strain RAF10 belonged to the genus Streptomyces. The comparison of its physiological characteristics with those of known species of Streptomyces showed some differences with the nearest species Streptomyces enissocaesilis. Analysis of the 16S rDNA sequence of strain RAF10 showed a similarity level ranging between 97.22 and 98.37% within Streptomyces species, with S. enissocaesilis the most closely related. However, the phylogenetic analysis indicated that strain RAF10 represent a distinct phyletic line suggesting a new genomic species. This novel strain was active against Gram-positive and Gram-negative Bacteria, yeasts and filamentous fungi. The highest antibiotic formation was obtained when using (ISP 4) broth medium with some modifications, for 120 h at 28 degree C in New Brunswick Scientific Shaker at 200 rpm.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1682-296X
DOI:10.3923/biotech.2007.489.496