Lack of alpha-1-adrenergic receptor-mediated downregulation of angiotensin II receptors in neuronal cultures from spontaneously hypertensive rat brain

Neuronal cells from Wistar Kyoto (WKY) and spontaneously hypertensive (SH) rat brains were established in culture to compare the expression of angiotensin II (Ang II) specific receptors and their regulation by norepinephrine (NE). Neurons from SH rat brains possess twice more Ang II specific recepto...

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Bibliographic Details
Published inMolecular and cellular biochemistry Vol. 91; no. 1-2; p. 111
Main Authors Raizada, M K, Sumners, C
Format Journal Article
LanguageEnglish
Published Netherlands 23.11.1989
Subjects
Animals
Cells, Cultured
Down-Regulation
Neurons - metabolism
Norepinephrine - metabolism
Rats
Rats, Inbred SHR - metabolism
Rats, Inbred Strains - metabolism
Rats, Inbred WKY
Receptors, Adrenergic, alpha - pharmacology
Receptors, Angiotensin - metabolism
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Summary:Neuronal cells from Wistar Kyoto (WKY) and spontaneously hypertensive (SH) rat brains were established in culture to compare the expression of angiotensin II (Ang II) specific receptors and their regulation by norepinephrine (NE). Neurons from SH rat brains possess twice more Ang II specific receptors and expressed a proportional increase in Ang II stimulated [3H]-NE uptake compared with WKY neurons. NE caused a dose-dependent decrease in 125I-Ang II binding in WKY neurons, an effect not observed when neurons from SH rat brains were incubated with NE. These observations suggest that the lack of NE-induced downregulation of Ang II receptors in neuronal cultures is genetically regulated.
ISSN:0300-8177
DOI:10.1007/BF00228085