Comparación de dos métodos de extracción de ADN a partir de plantas del género Solanum, subgénero Leptostemonum

Two methods were evaluated for ADN extraction in plants belonging to the Solanum genus. The objective was to isolate adequate ADN amount and of enough quality for obtaining sequences. The commercial product DNeasy® Plant Mini Kit was compared with a method that includes a lysis buffer. For the last...

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Bibliographic Details
Published inRevista Colombiana de biotecnologia Vol. 15; no. 2; p. 186
Main Authors Cadavid Sánchez, Isabel Cristina, Rosero García, Doris Amanda, Uribe Soto, Sandra Inés
Format Journal Article
LanguageEnglish
Spanish
Published Bogota Universidad Nacional de Colombia 01.12.2013
Subjects
Extraction processes
Flowers & plants
Genes
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Summary:Two methods were evaluated for ADN extraction in plants belonging to the Solanum genus. The objective was to isolate adequate ADN amount and of enough quality for obtaining sequences. The commercial product DNeasy® Plant Mini Kit was compared with a method that includes a lysis buffer. For the last method it was also evaluated the improvement when samples were previously macerated with liquid nitrogen. The quality results (A260/A280) did not show significant differences between the extraction methods (index <1.5). However, there were differences in the ADN concentration obtained (Dunnett test, p X2, p
ISSN:0123-3475
1909-8758
DOI:10.15446/rev.colomb.biote.v15n2.41747