Pressurized hot water extraction of 10-deacetylbaccatin III from yew for industrial application

• Published in: Resource-Efficient Technologies Vol. 3; no. 2; pp. 177 - 186
• Main Authors: Sixt, Maximilian, Strube, Jochen
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2017
• Subjects: Green solvents; Modeling; PHWE; Pressurized hot water extraction; Process design; Simulation; Yew; Process design; PHWE; Simulation; Green solvents; Pressurized hot water extraction; Yew; Modeling
• ISSN: 2405-6537
• DOI: 10.1016/j.reffit.2017.03.007

In this study a systematic and model-based approach for a process development focusing on pressurized hot water extraction (PHWE) is investigated, considering potential thermal degradation of high-value compounds. For extraction of 10-deacetylbaccatin III (10-DAB) from yew as a representative test system, water at 120 °C provided the best compromise between extraction yield and thermal degradation. A yield of almost 100% with regard to the overall amount of 10-DAB was reached in only 20 min. Each experiment for model parameter determination was carried out with 1.9 g of plant material at a flowrate of 1 mL/min and an applied pressure of 11 bar. All experimental values are assessed by a physico-chemical (rigorous) extraction model with experimental values and simulation results showing high conformity. In order to demonstrate the usability of the extraction model and model parameter determination a scale-up prediction was calculated. The scale-up experiments were predicted precisely and thus the model validated. The experiments and the simulation results for a column with a volume of 104 mL and a mass of 22 g yew needles were consistent with the milli-scale used for model parameter determination.