Chronic exposure to ozone induces cardiac antioxidant response and overexpression of either mitochondrial fision protein DRP1 and hipertrophyc-related proteins

• Published in: Journal of bioenergetics and biomembranes Vol. 54; no. 3; pp. 145 - 152
• Main Authors: Gómez-Crisóstomo, Nancy P., Rivas-Arancibia, Selva, Rodríguez-Martínez, Erika, De la Cruz-Hernández, Erick N., de María Márquez Álvarez, Corazón, Estrada Caraveo, Pablo Alexis, Gelista Herrera, Noemí, Valdés-Fuentes, Marlen, Martínez-Abundis, Eduardo
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2022; Springer Nature B.V
• Subjects: Actin; Air pollution; Animal Anatomy; Animal Biochemistry; Antioxidants; Apoptosis; Bax protein; Bcl-2 protein; Biochemistry; Bioorganic Chemistry; Cardiovascular diseases; Caspase-3; Caspase-9; Catalase; Chemistry; Chemistry and Materials Science; Chronic exposure; Contaminants; Exposure; Health risks; Heart; Histology; Hypertrophy; Markers; Mitochondria; Morphology; Organic Chemistry; Oxidative stress; Ozone; Proteins; Risk analysis; Risk factors; Superoxide dismutase; Cardiac hypertrophy; Oxidative stress; Ozone toxicity; Pollution; Mitochondrial dynamics
• ISSN: 0145-479X; 1573-6881
• DOI: 10.1007/s10863-022-09937-4

Pollution is considered a risk factor for cardiovascular disease; however, the mechanisms to explain this relationship are not well understood; ozone is one of the most abundant and studied air contaminants. Our study aimed to evaluate the effect of chronic exposition of rats to controlled low doses of ozone on oxidative stress, apoptosis, mitochondrial dynamics, and cardiac hypertrophy. Male Wistar rats were daily exposed to low ozone doses during 7, 15, 30, and 60 days, 4 h/day. Hearts were dissected, and homogenates were prepared. Oxidative stress was evaluated by TBARS and protein nitrosylation in addition to Superoxide dismutase 1 (SOD1) and Catalase levels; the apoptosis related-proteins caspase 3, caspase 9, Bax, Bcl-2, and the mitochondrial dynamic-associated proteins Fis1, Drp1, OPA1, and Mfn1 were quantified by western blot among the cardiac hypertrophy indicator alpha-actin (cardiac actin). There were no changes in the oxidative stress markers, however SOD1 expression increases. Caspase 3 expression decreased, whereas caspase 9 increased without changes in Bax or Bcl-2. Mitochondrial fission may be favored according to the increased expression of Drp1 but not changes in fusion-related proteins OPA1 and Mfn1. Finally, the molecular marker for cardiac hypertrophy was overexpressed after 30 and 60 days of ozone exposition. The chronic exposition to ozone induces a deleterious effect on cardiac mitochondria. Antioxidant defenses also show changes in relation to exposure time, as well as an apparent pro-hypertrophic effect associated with altered mitochondrial dynamics.