A revised model of regional cerebral ischemia by a middle cerebral artery occlusion in awake cat

• Published in: Japanese Journal of Stroke Vol. 9; no. 3; pp. 193 - 200
• Main Authors: Kuroiwa, Toshihiko, Tomida, Shuuichi, Seida, Mitsuru, Okeda, Riki, Inaba, Yutaka
• Format: Journal Article
• Language: Japanese
• Published: The Japan Stroke Society 1987
• Subjects: cerbral blood flow; focal cerebral ischemia; middle cerebral artery; recirculation
• ISSN: 0912-0726; 1883-1923
• DOI: 10.3995/jstroke.9.193

Since Sundt et al reported a retro-orbital MCA occlusion model, the model has been modified to trans-orbital approach and widely used in the experiments on cerebral ischemia. We modified the model in order to produce cerebral ischemia in awake state with minimum artificial change of intracranial pressure or leakage of CSF, and to be able to recirculate. For implantation of an MCA occlusion device, the MCA was exposed transorbitally and looped with nylon or prolene thread. The free ends of the thread was passed through two small holes of a small polyvinyl plate, placed loosely on the MCA, and then passed within a polypropyrene tube which was anchored to the orbit by dental cement after the craniotomy opening was sealed initially with gelfoam. The skin incision over the orbit was then sutured. Two to seven days after the implantation, the occlusion and recirculation of the MCA by our occlusion device was checked with cerebral angiography and rCBF measurement. Our modification of the MCA occlusion model to approximate clinical situations would be of value for studies on cerebral ischemia.