CD33 Detection by Immunohistochemistry in Paraffin-Embedded Tissues : A New Antibody Shows Excellent Specificity and Sensitivity for Cells of Myelomonocytic Lineage

A new monoclonal antibody to CD33 that reacts in paraffin-embedded tissue samples was evaluated. The expected reactivity in granulocytic and monocytic cells was found in a tissue microarray composed of multiple tissue sites. There was no unexpected reactivity found in a wide variety of hematolymphoi...

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Bibliographic Details
Published inAmerican journal of clinical pathology Vol. 129; no. 2; pp. 316 - 323
Main Authors HOYER, James D, GROGG, Karen L, HANSON, Curtis A, GAMEZ, Jeffrey D, DOGAN, Ahmet
Format Journal Article
LanguageEnglish
Published Chicago, IL American Society of Clinical Pathologists 01.02.2008
Subjects
Antibodies, Monoclonal
Antibody Specificity
Antigen-Antibody Reactions
Antigens, CD - immunology
Antigens, Differentiation, Myelomonocytic - immunology
Biological and medical sciences
Biomarkers
Bone Marrow - immunology
Flow Cytometry
Humans
Immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Leukemia, Myeloid - immunology
Medical sciences
Paraffin Embedding
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Precursor Cell Lymphoblastic Leukemia-Lymphoma - immunology
Reproducibility of Results
Sarcoma, Myeloid - diagnosis
Sarcoma, Myeloid - immunology
Sialic Acid Binding Ig-like Lectin 3
Tissue Array Analysis
Immunohistochemistry
Flow cytometry
Antibody
Acute leukemia
Cell lineage
Paraffin
Tissue
Anatomic pathology
Sensitivity
Specificity
Diagnosis
Detection
CD33
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Summary:A new monoclonal antibody to CD33 that reacts in paraffin-embedded tissue samples was evaluated. The expected reactivity in granulocytic and monocytic cells was found in a tissue microarray composed of multiple tissue sites. There was no unexpected reactivity found in a wide variety of hematolymphoid and nonhematolymphoid disorders. In cases of acute leukemia, the CD33 antibody showed equivalent results by immunohistochemical analysis compared with flow cytometric analysis. The CD33 antibody was also found to be a useful marker in the workup of myeloid sarcomas. This anti-CD33 antibody will be a useful marker in the workup of acute leukemias and myeloid sarcomas on paraffin-embedded tissue samples.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0002-9173
1943-7722
DOI:10.1309/E36008Y2H08Q1AYY