Eradication of exotoxin A and its producer in freshwater by means of cold-vaporized hydrogen peroxide-enhanced SDBD: A sustainable processing

• Published in: Aquaculture Vol. 559; p. 738380
• Main Authors: Sohbatzadeh, Farshad, Haqpanah, Hanieh, Shabannejad, Amir, Yazdanshenas, Homayoon
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.10.2022
• Subjects: Aquatic environments; Cold atmospheric plasma; Decontamination; Ecotoxicity; Exotoxin a; Gammarus roeseli; Pseudomonas aeruginosa; Gammarus roeseli; Decontamination; Aquatic environments; Ecotoxicity; Pseudomonas aeruginosa; Cold atmospheric plasma; Exotoxin a
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2022.738380

Pseudomonas aeruginosa is a multidrug-resistant bacterial strain with the ability to produce exotoxin A which can pose a serious threat to freshwater ecosystems by having pathogenicity against eukaryotes. Detoxification of exotoxin A and disinfection of P. aeruginosa are the main aims of this study. Using a high dosage of antibiotics might have more toxic effects on ecosystems while cold atmospheric plasma (CAP) can promise reliable, rapid, and environmentally friendly detoxification and disinfection. In this study we produced CAP reinforced by H2O2/H2O cold vapor to detoxify exotoxin A and inactivate P. aeruginosa in freshwater. We used Gammarus roeseli as the indicator of ecotoxicity in freshwater. The mortality of G. roeseli individuals elucidated that 420 s of CAP-based treatment under a surface dielectric barrier discharge (SDBD) set up can effectively passivize exotoxin A in freshwater. Meanwhile, a considerable decline in the concentration of exotoxin A was observed in treated samples due to the disruption of the protein structure. Ignorable side effects and changes to the physiochemical properties were observed. On the other hand, 8.2-log reduction of P. aeruginosa viable cells was observed after 300 s of treatment by our CAP-based strategy. This study also showed that the combination of hydrogen peroxide and CAP can significantly increase the decontamination power of hydrogen peroxide and our strategy can eliminate the need of using high concentrations of H2O2 which decreases environmental concerns. •Eliminate the threat of exotoxin A and P. aeruginosa to the freshwater environments utilizing a novel CAP-based strategy.•Investigating possible side effects of cold atmospheric plasma on aquatic environments and its ecotoxicity by G. roeseli as a biomarker.•Evaluating the concentration of RONS to clarify their contribution to disinfection and detoxification effects of developed CAP-based setup.•Demonstrating the significant effect of plasma and cold vapor of hydrogen peroxide combination on increasing the power of decontamination and eliminating the need of using high concentrations of H2O2.